Knowledge Management System of Institue of Mechanics, CAS
| Tumor-Specific Expression Detected with the Use of an Expression Vector Driven by Human Telomerase Reverse Transcriptase Gene Promoter | |
Yin JJ; Fu PF; Wang XB ; Jin G(靳刚); Xiu RJ; Xiu, RJ (reprint author), Peking Union Med Coll, Chinese Acad Med Sci, Inst Microcirculat, Beijing 100005, Peoples R China.
| |
| Source Publication | Journal of Laboratory and Clinical Medicine
 |
| 2004 | |
| Volume | 144Issue:6Pages:302-306 |
| ISSN | 0022-2143 |
| Abstract | To construct the tumor-specific expression vector driven by human telomerase reserve transcriptase gene promoter, we amplified a fragment of the enhanced green fluorescent protein (EGFP) gene from the pEGFP-N 1 plasmid and cloned it into the multiple cloning site of the pLNCX vector, then named the recombinant as pLNCX-EGFP. The fragment of human telomerase reverse transcriptase gene promoter (hTERT) was amplified from the human genome with the use of human telomerase reserve transcriptase gene-specific primers and cloned into the pLNCX-EGFP vector, from which the cytomegalovirus promoter had previously been removed through the use of restriction enzymes, in sense orientation relative to the green fluorescent protein coding sequence. Then the expression vector pLNT-EGFP-under the control of the human telomerase reserve transcriptase gene promoter, which contains green fluorescent protein reporter gene-was successfully constructed. To detect the transcriptional activity of the human telomerase reserve transcriptase gene promoter, we conducted transient transfection of this specific expression vector into human lung fibroblast (HLF) cell lines with high telomerase activity and normal human fetal lung fibroblast (W138) cell lines without telomerase activity. The results of transient transfection showed that the pLNT-EGFP vector strongly expressed the green fluorescent protein reporter gene in telomerase-positive cells but not in telomerase-negative cells. |
| Subject Area | 力学 |
| DOI | 10.1016/j.lab.2004.09.010 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000225891100004 |
| WOS Keyword | CANCER-CELLS ; CLONING ; HTERT |
| WOS Research Area | Medical Laboratory Technology ; General & Internal Medicine ; Research & Experimental Medicine |
| WOS Subject | Medical Laboratory Technology ; Medicine, General & Internal ; Medicine, Research & Experimental |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/15334 |
| Collection | 力学所知识产出(1956-2008) |
| Corresponding Author | Xiu, RJ (reprint author), Peking Union Med Coll, Chinese Acad Med Sci, Inst Microcirculat, Beijing 100005, Peoples R China. |
| Recommended Citation GB/T 7714 | Yin JJ,Fu PF,Wang XB,et al. Tumor-Specific Expression Detected with the Use of an Expression Vector Driven by Human Telomerase Reverse Transcriptase Gene Promoter[J]. Journal of Laboratory and Clinical Medicine,2004,144,6,:302-306. |
| APA | Yin JJ,Fu PF,Wang XB,靳刚,Xiu RJ,&Xiu, RJ .(2004).Tumor-Specific Expression Detected with the Use of an Expression Vector Driven by Human Telomerase Reverse Transcriptase Gene Promoter.Journal of Laboratory and Clinical Medicine,144(6),302-306. |
| MLA | Yin JJ,et al."Tumor-Specific Expression Detected with the Use of an Expression Vector Driven by Human Telomerase Reverse Transcriptase Gene Promoter".Journal of Laboratory and Clinical Medicine 144.6(2004):302-306. |
| Files in This Item: | Download All | |||||
| File Name/Size | DocType | Version | Access | License | ||
| Tumor-specific.pdf（293KB） | 开放获取 | -- | View Download | |||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment