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| Quantifying the Effects of Molecular Orientation and Length on Two-Dimensional Receptor-Ligand Binding Kinetics | |
Huang J ; Chen J; Chesla SE; Yago T; Mehta P; McEver RP; Zhu C; Long M(龙勉); Zhu, C (reprint author), Georgia Inst Technol, George W Woodruff Sch Mech Engn, Atlanta, GA 30332 USA.
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| Source Publication | Journal of Biological Chemistry
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| 2004 | |
| Volume | 279Issue:43Pages:44915-44923 |
| ISSN | 0021-9258 |
| Abstract | Surface presentation of adhesion receptors influences cell adhesion, although the mechanisms underlying these effects are not well understood. We used a micropipette adhesion frequency assay to quantify how the molecular orientation and length of adhesion receptors on the cell membrane affected two-dimensional kinetic rates of interactions with surface ligands. Interactions of P-selectin, E-selectin, and CD16A with their respective ligands or antibody were used to demonstrate such effects. Randomizing the orientation of the adhesion receptor or lowering its ligand- and antibody-binding domain above the cell membrane lowered two-dimensional affinities of the molecular interactions by reducing the forward rates but not the reverse rates. In contrast, the soluble antibody bound with similar three-dimensional affinities to cell-bound P-selectin constructs regardless of their orientation and length. These results demonstrate that the orientation and length of an adhesion receptor influences its rate of encountering and binding a surface ligand but does not subsequently affect the stability of binding. |
| Subject Area | 力学 |
| DOI | 10.1074/jbc.M407039200 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000224505600082 |
| WOS Keyword | SELECTIN GLYCOPROTEIN LIGAND-1 ; P-SELECTIN ; CELL-ADHESION ; CONCURRENT BINDING ; MEMBRANE ; FLOW ; NEUTROPHILS ; FIBRONECTIN ; AFFINITY ; IDENTIFICATION |
| WOS Research Area | Biochemistry & Molecular Biology |
| WOS Subject | Biochemistry & Molecular Biology |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/15716 |
| Collection | 力学所知识产出(1956-2008) |
| Corresponding Author | Zhu, C (reprint author), Georgia Inst Technol, George W Woodruff Sch Mech Engn, Atlanta, GA 30332 USA. |
| Recommended Citation GB/T 7714 | Huang J,Chen J,Chesla SE,et al. Quantifying the Effects of Molecular Orientation and Length on Two-Dimensional Receptor-Ligand Binding Kinetics[J]. Journal of Biological Chemistry,2004,279,43,:44915-44923. |
| APA | Huang J.,Chen J.,Chesla SE.,Yago T.,Mehta P.,...&Zhu, C .(2004).Quantifying the Effects of Molecular Orientation and Length on Two-Dimensional Receptor-Ligand Binding Kinetics.Journal of Biological Chemistry,279(43),44915-44923. |
| MLA | Huang J,et al."Quantifying the Effects of Molecular Orientation and Length on Two-Dimensional Receptor-Ligand Binding Kinetics".Journal of Biological Chemistry 279.43(2004):44915-44923. |
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| M407039200v1.pdf（401KB） | 开放获取 | -- | View Download | |||
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