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| Measuring Receptor/Ligand Interaction at the Single-Bond Level: Experimental and Interpretative Issues | |
Zhu C(朱承); Long M(龙勉) ; Chesla SE; Bongrand P; Zhu, C (reprint author), Georgia Inst Technol, George W Woodruff Sch Mech Engn, Atlanta, GA 30332 USA.
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| Source Publication | Annals of Biomedical Engineering
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| 2002 | |
| Volume | 30Issue:3Pages:305-314 |
| ISSN | 0090-6964 |
| Abstract | There is increased interest in measuring kinetic rates, lifetimes, and rupture forces of single receptor/ligand bonds. Valuable insights have been obtained from previous experiments attempting such measurements. However, it remains difficult to know with sufficient certainty that single bonds were indeed measured. Using exemplifying data, evidence supporting single-bond observation is examined and caveats in the experimental design and data interpretation are identified. Critical issues preventing definitive proof and disproof of single-bond observation include complex binding schemes, multimeric interactions, clustering, and heterogeneous surfaces. It is concluded that no single criterion is sufficient to ensure that single bonds are actually observed. However, a cumulative body of evidence may provide reasonable confidence. 0 2002 Biomedical Engineering Society. |
| Subject Area | 力学 |
| DOI | 10.1114/1.1467923 |
| Indexed By | SCI ; EI |
| Language | 英语 |
| WOS ID | WOS:000175849500004 |
| WOS Keyword | SELECTIN GLYCOPROTEIN LIGAND-1 ; ATOMIC-FORCE MICROSCOPY ; SHEAR-FLOW ; CELL-ADHESION ; P-SELECTIN ; MOLECULAR ADHESION ; KINETIC RATES ; BINDING ; LIFETIME ; MEMBRANE |
| WOS Research Area | Engineering |
| WOS Subject | Engineering, Biomedical |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/15995 |
| Collection | 力学所知识产出(1956-2008) |
| Corresponding Author | Zhu, C (reprint author), Georgia Inst Technol, George W Woodruff Sch Mech Engn, Atlanta, GA 30332 USA. |
| Recommended Citation GB/T 7714 | Zhu C,Long M,Chesla SE,et al. Measuring Receptor/Ligand Interaction at the Single-Bond Level: Experimental and Interpretative Issues[J]. Annals of Biomedical Engineering,2002,30,3,:305-314. |
| APA | 朱承,龙勉,Chesla SE,Bongrand P,&Zhu, C .(2002).Measuring Receptor/Ligand Interaction at the Single-Bond Level: Experimental and Interpretative Issues.Annals of Biomedical Engineering,30(3),305-314. |
| MLA | 朱承,et al."Measuring Receptor/Ligand Interaction at the Single-Bond Level: Experimental and Interpretative Issues".Annals of Biomedical Engineering 30.3(2002):305-314. |
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