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| 抗SARS人源单链抗体H12的表达及复性 | |
| Alternative Title | Expression and Renaturation of a Novel Human Single-chain Fv Antibody Against SARS-CoV |
段金柱; 齐财; 韩伟; 王战会; 靳刚 ; 阎锡蕴
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| Source Publication | 生物工程学报 |
| 2005 | |
| Volume | 21Issue:5Pages:692-697 |
| ISSN | 1000-3061 |
| Abstract | 从SARS免疫抗体库获得的一株抗SARS-CoV人源单链抗体H12,亟待鉴定.为了快速制备大量具有生物活性的单链抗体H12,构建了pET28a-H12原核高表达载体,表达量占菌体总蛋白质30%以上.采用稀释复性和分子筛柱复性两种方法对包涵体蛋白进行复性与纯化,结果显示两种方法都能使得单链抗体复性.与稀释复性法相比,柱复性效果更好,其抗原结合活性是稀释复性法的1.51倍.柱复性后的单链抗体亲和力测定的解离常数Kd为73.5nmol/mL.为进一步研究单链抗体H12的功能奠定了基础. |
| Keyword | 单链抗体 原核表达 包涵体 复性 Sars-cov |
| Subject Area | 力学 |
| Indexed By | CSCD |
| Language | 中文 |
| CSCD ID | CSCD:2043984 |
| Citation statistics |
Cited Times:2[CSCD]
[CSCD Record]
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| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/16067 |
| Collection | 力学所知识产出(1956-2008) |
| Recommended Citation GB/T 7714 | 段金柱,齐财,韩伟,等. 抗SARS人源单链抗体H12的表达及复性[J]. 生物工程学报,2005,21,5,:692-697. |
| APA | 段金柱,齐财,韩伟,王战会,靳刚,&阎锡蕴.(2005).抗SARS人源单链抗体H12的表达及复性.生物工程学报,21(5),692-697. |
| MLA | 段金柱,et al."抗SARS人源单链抗体H12的表达及复性".生物工程学报 21.5(2005):692-697. |
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