Knowledge Management System of Institue of Mechanics, CAS
| Application of Optical Protein-chip in Detecting Phage M13KO7 | |
| Alternative Title | 光学蛋白芯片技术在噬菌体M13KO7检测中的应用 |
Qi C(齐财); Feng J(冯静) ; Wang ZH(王战会); Meng YH(孟永宏); Yan XY(阎锡蕴); Jin G(靳刚)
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| Source Publication | CHINESE JOURNAL OF BIOTECHNOLOGY,2006,
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| 2006 | |
| Volume | 22Issue:5Pages:856-860 |
| ISSN | 1000-3061 |
| Abstract | Abstract: Avidin layer was bound on the substrate surface of Silicon wafer modified with aldehyde. The interaction between avidin and biotin was adopted for the immobilization of mouse monoclonal biotin-anti-M13 (antibody GP3)-labeled biotin. The surface was incubated in a solution containing phage M13KO7, which was trapped by the antibody GP3 with the interaction between phage M13KO7 and antibody GP3, resulting in a variation of layer thickness that was detected by imaging ellipsometry. The results showed a saturated layer of antibody GP3 with a thickness about 6.9 nm on the surface of the silicon wafer. The specific interaction between phage M13KO7 and antibody GP3 resulted in a variation of layer thickness. The layer of phage M13KO7 bound with antibody GP3 was 17.5 nm in the concentration of 1.1×1010 pfu/mL. Each variation of the layer thickness corresponded to a concentration of phage M13KO7 in the range of 0.1×1010–2.5×1010 pfu/mL, with the sensitivity of 109 pfu/mL. Compared with other methods, the optical protein-chip, requiring only short measurement time, label free, is a quantitative test, and can be visualized. This study could be significant on the interactions between the antibody and the virus, showing potential in the early diagnosis of virosis. |
| Subject Area | 力学 |
| Indexed By | 其他 |
| Language | 英语 |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/17293 |
| Collection | 力学所知识产出(1956-2008) |
| Recommended Citation GB/T 7714 | Qi C,Feng J,Wang ZH,et al. Application of Optical Protein-chip in Detecting Phage M13KO7[J]. CHINESE JOURNAL OF BIOTECHNOLOGY,2006,,2006,22,5,:856-860. |
| APA | Qi C,Feng J,Wang ZH,Meng YH,Yan XY,&Jin G.(2006).Application of Optical Protein-chip in Detecting Phage M13KO7.CHINESE JOURNAL OF BIOTECHNOLOGY,2006,,22(5),856-860. |
| MLA | Qi C,et al."Application of Optical Protein-chip in Detecting Phage M13KO7".CHINESE JOURNAL OF BIOTECHNOLOGY,2006, 22.5(2006):856-860. |
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