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| Immunosensor Interface Based on Physical and Chemical Immunogylobulin G Adsorption onto Mixed Self-Assembled Monolayers | |
Wang ZH(王战会); Viana AS; Jin G(靳刚) ; Abrantes LM; Abrantes, LM (reprint author), Univ Lisbon, Fac Ciencias, Lab SPM, Campo Grande, P-1749016 Lisbon, Portugal.
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| Source Publication | Bioelectrochemistry
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| 2006-10 | |
| Volume | 69Issue:2Pages:180-186 |
| ISSN | 1567-5394 |
| Abstract | An immunosensor interface based on mixed hydrophobic self-assembled monolayers (SAMs) of methyl and carboxylic acid terminated thiols with covalently attached human Immunoglobulin G (hIgG), is investigated. The densely packed and organised SAMs were characterised by contact angle measurements and cyclic voltammetry. The effect of the non-ionic surfactant, Tween 20, in preventing nonspecific adsorption is addressed by ellipsometry during physical and covalent hIgG immobilization on pure and mixed SAMs, respectively. It is clearly demonstrated that nonspecific adsorption due to hydrophobic interactions of hIgG on methyl ended groups is totally inhibited, whereas electrostatic/hydrogen bonding interactions with the exposed carboxylic groups prevail in the presence of surfactant. Results of ellipsometry and Atomic Force Microscopy, reveal that the surface concentration of covalently immobilized hIgG is determined by the ratio of COOH/CH3-terminated thiols in SAM forming solution. Moreover, the ellipsometric data demonstrates that the ratio of bound anti-hIgG/hIgG depends on the density of hIgG on the surface and that the highest ratio is close to three. We also report the selectivity and high sensitivity achieved by chronoamperometry in the detection of adsorbed hIgG and the reaction with its antibody. |
| Keyword | Mixed Self-assembled Monolayers Immunosensor Human Immunoglobulin g Tween 20 Electrochemistry Ellipsometry |
| Subject Area | 力学 |
| DOI | 10.1016/j.bioelechem.2006.02.001 |
| URL | 查看原文 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000242005800007 |
| WOS Keyword | SURFACE-PLASMON RESONANCE ; SCANNING FORCE MICROSCOPY ; PROTEIN ADSORPTION ; ALKANETHIOLATE MONOLAYERS ; POLY(ETHYLENE GLYCOL) ; REDUCTIVE DESORPTION ; GOLD ELECTRODES ; MODEL SURFACES ; IMMOBILIZATION ; ANTIBODY |
| WOS Research Area | Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics ; Biophysics ; Electrochemistry |
| WOS Subject | Biochemistry & Molecular Biology ; Biology ; Biophysics ; Electrochemistry |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/17676 |
| Collection | 力学所知识产出(1956-2008) |
| Corresponding Author | Abrantes, LM (reprint author), Univ Lisbon, Fac Ciencias, Lab SPM, Campo Grande, P-1749016 Lisbon, Portugal. |
| Recommended Citation GB/T 7714 | Wang ZH,Viana AS,Jin G,et al. Immunosensor Interface Based on Physical and Chemical Immunogylobulin G Adsorption onto Mixed Self-Assembled Monolayers[J]. Bioelectrochemistry,2006,69,2,:180-186. |
| APA | Wang ZH,Viana AS,Jin G,Abrantes LM,&Abrantes, LM .(2006).Immunosensor Interface Based on Physical and Chemical Immunogylobulin G Adsorption onto Mixed Self-Assembled Monolayers.Bioelectrochemistry,69(2),180-186. |
| MLA | Wang ZH,et al."Immunosensor Interface Based on Physical and Chemical Immunogylobulin G Adsorption onto Mixed Self-Assembled Monolayers".Bioelectrochemistry 69.2(2006):180-186. |
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