Quantifying Cell Binding Kinetics Mediated By Surface-Bound Blood Type B Antigen To Immobilized Antibodies

doi:10.1007/s11434-008-0517-9

IMECH-IR > 力学所知识产出(1956-2008)

	Quantifying Cell Binding Kinetics Mediated By Surface-Bound Blood Type B Antigen To Immobilized Antibodies
	Li BX(李宝霞); Chen J(陈娟); Long M(龙勉); Long, M (reprint author), Chinese Acad Sci, Inst Mech, Natl Micrograv Lab, Beijing 100190, Peoples R China.
发表期刊	Chinese Science Bulletin/中国科学通报(英文版)
	2008
页码	3634-3641
ISSN	1001-6538
摘要	Cell adhesion is crucial to many biological processes, such as inflammatory responses, tumor metastasis and thrombosis formation. Recently a commercial surface plasmon resonance (SPR)-based BIAcore biosensor has been extended to determine cell binding mediated by surface-bound biomolecular interactions. How such cell binding is quantitatively governed by kinetic rates and regulating factors, however, has been poorly understood. Here we developed a novel assay to determine the binding kinetics of surface-bound biomolecular interactions using a commercial BIAcore 3000 biosensor. Human red blood cells (RBCs) presenting blood group B antigen and CM5 chip bearing immobilized anti-B monoclonal antibody (mAb) were used to obtain the time courses of response unit, or sensorgrams, when flowing RBCs over the chip surface. A cellular kinetic model was proposed to correlate the sensorgrams with kinetic rates. Impacts of regulating factors, such as cell concentration, flow duration and rate, antibody-presenting level, as well as pH value and osmotic pressure of suspending medium were tested systematically, which imparted the confidence that the approach can be applied to kinetic measurements of cell adhesion mediated by surface-bound biomolecular interactions. These results provided a new insight into quantifying cell binding using a commercial SPR-based BIAcore biosensor.
关键词	Biacore Biosensor Response Unit Cellular Kinetic Model Cellular Off-rate Cellular On-rate Plasmon Resonance Listeria-monocytogenes Mass-transport Rate Constants Monoclonal-antibody Optical Biosensor Small Systems Affinity Equilibrium Technology
DOI	10.1007/s11434-008-0517-9
收录类别	SCI
语种	英语
WOS记录号	WOS:000261257600005
关键词[WOS]	PLASMON RESONANCE ; LISTERIA-MONOCYTOGENES ; MASS-TRANSPORT ; RATE CONSTANTS ; MONOCLONAL-ANTIBODY ; OPTICAL BIOSENSOR ; SMALL SYSTEMS ; AFFINITY ; EQUILIBRIUM ; TECHNOLOGY
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/25818
专题	力学所知识产出(1956-2008)
通讯作者	Long, M (reprint author), Chinese Acad Sci, Inst Mech, Natl Micrograv Lab, Beijing 100190, Peoples R China.
推荐引用方式 GB/T 7714	Li BX,Chen J,Long M,et al. Quantifying Cell Binding Kinetics Mediated By Surface-Bound Blood Type B Antigen To Immobilized Antibodies[J]. Chinese Science Bulletin/中国科学通报(英文版),2008:3634-3641.
APA	李宝霞,陈娟,龙勉,&Long, M .(2008).Quantifying Cell Binding Kinetics Mediated By Surface-Bound Blood Type B Antigen To Immobilized Antibodies.Chinese Science Bulletin/中国科学通报(英文版),3634-3641.
MLA	李宝霞,et al."Quantifying Cell Binding Kinetics Mediated By Surface-Bound Blood Type B Antigen To Immobilized Antibodies".Chinese Science Bulletin/中国科学通报(英文版) (2008):3634-3641.

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