Knowledge Management System of Institue of Mechanics, CAS
| Quantifying Cell Binding Kinetics Mediated By Surface-Bound Blood Type B Antigen To Immobilized Antibodies | |
Li BX(李宝霞) ; Chen J(陈娟); Long M(龙勉); Long, M (reprint author), Chinese Acad Sci, Inst Mech, Natl Micrograv Lab, Beijing 100190, Peoples R China.
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| Source Publication | Chinese Science Bulletin/中国科学通报(英文版)
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| 2008 | |
| Pages | 3634-3641 |
| ISSN | 1001-6538 |
| Abstract | Cell adhesion is crucial to many biological processes, such as inflammatory responses, tumor metastasis and thrombosis formation. Recently a commercial surface plasmon resonance (SPR)-based BIAcore biosensor has been extended to determine cell binding mediated by surface-bound biomolecular interactions. How such cell binding is quantitatively governed by kinetic rates and regulating factors, however, has been poorly understood. Here we developed a novel assay to determine the binding kinetics of surface-bound biomolecular interactions using a commercial BIAcore 3000 biosensor. Human red blood cells (RBCs) presenting blood group B antigen and CM5 chip bearing immobilized anti-B monoclonal antibody (mAb) were used to obtain the time courses of response unit, or sensorgrams, when flowing RBCs over the chip surface. A cellular kinetic model was proposed to correlate the sensorgrams with kinetic rates. Impacts of regulating factors, such as cell concentration, flow duration and rate, antibody-presenting level, as well as pH value and osmotic pressure of suspending medium were tested systematically, which imparted the confidence that the approach can be applied to kinetic measurements of cell adhesion mediated by surface-bound biomolecular interactions. These results provided a new insight into quantifying cell binding using a commercial SPR-based BIAcore biosensor. |
| Keyword | Biacore Biosensor Response Unit Cellular Kinetic Model Cellular Off-rate Cellular On-rate Plasmon Resonance Listeria-monocytogenes Mass-transport Rate Constants Monoclonal-antibody Optical Biosensor Small Systems Affinity Equilibrium Technology |
| DOI | 10.1007/s11434-008-0517-9 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000261257600005 |
| WOS Keyword | PLASMON RESONANCE ; LISTERIA-MONOCYTOGENES ; MASS-TRANSPORT ; RATE CONSTANTS ; MONOCLONAL-ANTIBODY ; OPTICAL BIOSENSOR ; SMALL SYSTEMS ; AFFINITY ; EQUILIBRIUM ; TECHNOLOGY |
| WOS Research Area | Science & Technology - Other Topics |
| WOS Subject | Multidisciplinary Sciences |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/25818 |
| Collection | 力学所知识产出(1956-2008) |
| Corresponding Author | Long, M (reprint author), Chinese Acad Sci, Inst Mech, Natl Micrograv Lab, Beijing 100190, Peoples R China. |
| Recommended Citation GB/T 7714 | Li BX,Chen J,Long M,et al. Quantifying Cell Binding Kinetics Mediated By Surface-Bound Blood Type B Antigen To Immobilized Antibodies[J]. Chinese Science Bulletin/中国科学通报(英文版),2008:3634-3641. |
| APA | 李宝霞,陈娟,龙勉,&Long, M .(2008).Quantifying Cell Binding Kinetics Mediated By Surface-Bound Blood Type B Antigen To Immobilized Antibodies.Chinese Science Bulletin/中国科学通报(英文版),3634-3641. |
| MLA | 李宝霞,et al."Quantifying Cell Binding Kinetics Mediated By Surface-Bound Blood Type B Antigen To Immobilized Antibodies".Chinese Science Bulletin/中国科学通报(英文版) (2008):3634-3641. |
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