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| Two-Dimensional Kinetics Of Beta(2)-Integrin And Icam-1 Bindings Between Neutrophils And Melanoma Cells In A Shear Flow | |
Liang SL; Fu ZL(傅长亮) ; Wagner D; Guo HG; Zhan DY; Dong C; Long M(龙勉); Dong, C (reprint author), Penn State Univ, Dept Bioengn, University Pk, PA 16802 USA.
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| Source Publication | American Journal of Physiology-Cell Physiology
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| 2008 | |
| Pages | C743-C753 |
| ISSN | 0363-6143 |
| Abstract | Cell adhesion, mediated by specific receptor-ligand interactions, plays an important role in biological processes such as tumor metastasis and inflammatory cascade. For example, interactions between beta(2)-integrin ( lymphocyte function-associated antigen-1 and/or Mac-1) on polymorphonuclear neutrophils (PMNs) and ICAM-1 on melanoma cells initiate the bindings of melanoma cells to PMNs within the tumor microenvironment in blood flow, which in turn activate PMN-melanoma cell aggregation in a near-wall region of the vascular endothelium, therefore enhancing subsequent extravasation of melanoma cells in the microcirculations. Kinetics of integrin-ligand bindings in a shear flow is the determinant of such a process, which has not been well understood. In the present study, interactions of PMNs with WM9 melanoma cells were investigated to quantify the kinetics of beta(2)-integrin and ICAM-1 bindings using a cone-plate viscometer that generates a linear shear flow combined with a two-color flow cytometry technique. Aggregation fractions exhibited a transition phase where it first increased before 60 s and then decreased with shear durations. Melanoma-PMN aggregation was also found to be inversely correlated with the shear rate. A previously developed probabilistic model was modified to predict the time dependence of aggregation fractions at different shear rates and medium viscosities. Kinetic parameters of beta(2)-integrin and ICAM-1 bindings were obtained by individual or global fittings, which were comparable to respectively published values. These findings provide new quantitative understanding of the biophysical basis of leukocyte-tumor cell interactions mediated by specific receptor-ligand interactions under shear flow conditions. |
| Keyword | Heterotypic Cell Aggregation Adhesion Molecule Leukocyte Tumor Cell Reverse Rate Binding Affinity Probabilistic Model Polymorpho-nuclear Neutrophils Intercellular Adhesion Molecule-1 Intercellular-adhesion Molecule-1 Ligand-mediated Adhesion Hydrodynamic Shear L-selectin Malignant-melanoma Dynamic Regulation Variant Isoforms Stable Adhesion Latex Spheres P-selectin |
| DOI | 10.1152/ajpcell.00250.2007 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000253992300009 |
| WOS Keyword | INTERCELLULAR-ADHESION MOLECULE-1 ; LIGAND-MEDIATED ADHESION ; HYDRODYNAMIC SHEAR ; L-SELECTIN ; MALIGNANT-MELANOMA ; DYNAMIC REGULATION ; VARIANT ISOFORMS ; STABLE ADHESION ; LATEX SPHERES ; INTEGRIN |
| WOS Research Area | Cell Biology ; Physiology |
| WOS Subject | Cell Biology ; Physiology |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/26114 |
| Collection | 力学所知识产出(1956-2008) |
| Corresponding Author | Dong, C (reprint author), Penn State Univ, Dept Bioengn, University Pk, PA 16802 USA. |
| Recommended Citation GB/T 7714 | Liang SL,Fu ZL,Wagner D,et al. Two-Dimensional Kinetics Of Beta(2)-Integrin And Icam-1 Bindings Between Neutrophils And Melanoma Cells In A Shear Flow[J]. American Journal of Physiology-Cell Physiology,2008:C743-C753. |
| APA | Liang SL.,傅长亮.,Wagner D.,Guo HG.,Zhan DY.,...&Dong, C .(2008).Two-Dimensional Kinetics Of Beta(2)-Integrin And Icam-1 Bindings Between Neutrophils And Melanoma Cells In A Shear Flow.American Journal of Physiology-Cell Physiology,C743-C753. |
| MLA | Liang SL,et al."Two-Dimensional Kinetics Of Beta(2)-Integrin And Icam-1 Bindings Between Neutrophils And Melanoma Cells In A Shear Flow".American Journal of Physiology-Cell Physiology (2008):C743-C753. |
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