| Parametric Analysis for Monitoring 2D Kinetics of Receptor-Ligand binding | |
Sun GY(孙淦云) ; Zhang Y(章燕); Huo B(霍波); Long M(龙勉)
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| Source Publication | Cellular and Molecular Bioengineering
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| 2009 | |
| Volume | 2Issue:4Pages:495-503 |
| ISSN | 1865-5025 |
| Abstract | Thermal fluctuation approach is widely used to monitor association kinetics of surface-bound receptor-ligand interactions. Various protocols such as sliding standard deviation (SD) analysis (SSA) and Page's test analysis (PTA) have been used to estimate two-dimensional (2D) kinetic rates from the time course of displacement of molecular carrier. In the current work, we compared the estimations from both SSA and modified PTA using measured data from an optical trap assay and simulated data from a random number generator. Our results indicated that both SSA and PTA were reliable in estimating 2D kinetic rates. Parametric analysis also demonstrated that such the estimations were sensitive to parameters such as sampling rate, sliding window size, and threshold. These results furthered the understandings in quantifying the biophysics of receptor-ligand interactions. |
| Subject Area | 生物力学 |
| DOI | 10.1007/s12195-009-0079-1 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000272671600005 |
| WOS Keyword | L-SELECTIN ; ADHESION MOLECULES ; OPTICAL TWEEZERS ; P-SELECTIN ; FORCE ; BONDS ; SHEAR ; FLOW ; FLUCTUATIONS ; DISSOCIATION |
| WOS Research Area | Cell Biology ; Biophysics |
| WOS Subject | Cell & Tissue Engineering ; Biophysics ; Cell Biology |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/33023 |
| Collection | 微重力重点实验室 |
| Corresponding Author | Long M(龙勉) |
| Recommended Citation GB/T 7714 | Sun GY,Zhang Y,Huo B,et al. Parametric Analysis for Monitoring 2D Kinetics of Receptor-Ligand binding[J]. Cellular and Molecular Bioengineering,2009,2,4,:495-503. |
| APA | 孙淦云,章燕,霍波,&龙勉.(2009).Parametric Analysis for Monitoring 2D Kinetics of Receptor-Ligand binding.Cellular and Molecular Bioengineering,2(4),495-503. |
| MLA | 孙淦云,et al."Parametric Analysis for Monitoring 2D Kinetics of Receptor-Ligand binding".Cellular and Molecular Bioengineering 2.4(2009):495-503. |
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