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| Adsorption of human serum albumin onto gold: a combined electrochemical and ellipsometric study | |
Ying PQ(应佩青); Viana AS; Abrantes LM; Jin G(靳刚) ; Jin, G (reprint author), Chinese Acad Sci, Inst Mech, Beijing 100080, Peoples R China.
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| Source Publication | Journal of Colloid and Interface Science
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| 2004 | |
| Volume | 279Issue:1Pages:95-99 |
| ISSN | 0021-9797 |
| Abstract | Human serum albumin adsorption onto gold surfaces was investigated by electrochemical and ellipsometric methods. Albumin adsorption onto gold was confirmed by the change of the open circuit potential of gold and by the ellipsometric parameter variation during albumin immobilization. In both experiments the parameters reached stable values within 10-15 min. The albumin adsorption layer thickness measured with the ellipsometer was about 1.5 nm. The adsorption of albumin Under applied potential was also investigated and it was found that both positive and negative applied potential promote albumin adsorption. Changes in the optical parameters of bare gold and albumin adsorbed onto gold surface under applied potential were investigated with in Situ ellipsometry. The similarity and reversibility of the optical changes showed that adsorbed albumin was stable on the gold surface Under the applied potential range (-200-600 mV). The cyclic voltammograms of K3Fe(CN)(6) on the modified gold surface showed that albumin Could partly block the oxidation and reduction reaction. (C) 2004 Elsevier Inc. All rights reserved. |
| Keyword | Human Serum Albumin Adsorption Gold Electrochemistry Ellipsometry Substratum Surface-properties Protein Adsorption Metal-surfaces Electrode Reflectometry Fibrinogen Interface Layers |
| DOI | 10.1016/j.jcis.2004.06.048 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000224708300010 |
| WOS Keyword | SUBSTRATUM SURFACE-PROPERTIES ; PROTEIN ADSORPTION ; METAL-SURFACES ; ELECTRODE ; REFLECTOMETRY ; FIBRINOGEN ; INTERFACE ; LAYERS |
| WOS Research Area | Chemistry |
| WOS Subject | Chemistry, Physical |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/33749 |
| Collection | 力学所知识产出(1956-2008) |
| Corresponding Author | Jin, G (reprint author), Chinese Acad Sci, Inst Mech, Beijing 100080, Peoples R China. |
| Recommended Citation GB/T 7714 | Ying PQ,Viana AS,Abrantes LM,et al. Adsorption of human serum albumin onto gold: a combined electrochemical and ellipsometric study[J]. Journal of Colloid and Interface Science,2004,279,1,:95-99. |
| APA | 应佩青,Viana AS,Abrantes LM,靳刚,&Jin, G .(2004).Adsorption of human serum albumin onto gold: a combined electrochemical and ellipsometric study.Journal of Colloid and Interface Science,279(1),95-99. |
| MLA | 应佩青,et al."Adsorption of human serum albumin onto gold: a combined electrochemical and ellipsometric study".Journal of Colloid and Interface Science 279.1(2004):95-99. |
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