Adsorption of human serum albumin onto gold: a combined electrochemical and ellipsometric study

doi:10.1016/j.jcis.2004.06.048

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	Adsorption of human serum albumin onto gold: a combined electrochemical and ellipsometric study
	Ying PQ(应佩青); Viana AS; Abrantes LM; Jin G(靳刚); Jin, G (reprint author), Chinese Acad Sci, Inst Mech, Beijing 100080, Peoples R China.
发表期刊	Journal of Colloid and Interface Science
	2004
卷号	279 期号:1 页码:95-99
ISSN	0021-9797
摘要	Human serum albumin adsorption onto gold surfaces was investigated by electrochemical and ellipsometric methods. Albumin adsorption onto gold was confirmed by the change of the open circuit potential of gold and by the ellipsometric parameter variation during albumin immobilization. In both experiments the parameters reached stable values within 10-15 min. The albumin adsorption layer thickness measured with the ellipsometer was about 1.5 nm. The adsorption of albumin Under applied potential was also investigated and it was found that both positive and negative applied potential promote albumin adsorption. Changes in the optical parameters of bare gold and albumin adsorbed onto gold surface under applied potential were investigated with in Situ ellipsometry. The similarity and reversibility of the optical changes showed that adsorbed albumin was stable on the gold surface Under the applied potential range (-200-600 mV). The cyclic voltammograms of K3Fe(CN)(6) on the modified gold surface showed that albumin Could partly block the oxidation and reduction reaction. (C) 2004 Elsevier Inc. All rights reserved.
关键词	Human Serum Albumin Adsorption Gold Electrochemistry Ellipsometry Substratum Surface-properties Protein Adsorption Metal-surfaces Electrode Reflectometry Fibrinogen Interface Layers
DOI	10.1016/j.jcis.2004.06.048
收录类别	SCI
语种	英语
WOS记录号	WOS:000224708300010
关键词[WOS]	SUBSTRATUM SURFACE-PROPERTIES ; PROTEIN ADSORPTION ; METAL-SURFACES ; ELECTRODE ; REFLECTOMETRY ; FIBRINOGEN ; INTERFACE ; LAYERS
WOS研究方向	Chemistry
WOS类目	Chemistry, Physical
引用统计	被引频次：42[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/33749
专题	力学所知识产出(1956-2008)
通讯作者	Jin, G (reprint author), Chinese Acad Sci, Inst Mech, Beijing 100080, Peoples R China.
推荐引用方式 GB/T 7714	Ying PQ,Viana AS,Abrantes LM,et al. Adsorption of human serum albumin onto gold: a combined electrochemical and ellipsometric study[J]. Journal of Colloid and Interface Science,2004,279,1,:95-99.
APA	应佩青,Viana AS,Abrantes LM,靳刚,&Jin, G .(2004).Adsorption of human serum albumin onto gold: a combined electrochemical and ellipsometric study.Journal of Colloid and Interface Science,279(1),95-99.
MLA	应佩青,et al."Adsorption of human serum albumin onto gold: a combined electrochemical and ellipsometric study".Journal of Colloid and Interface Science 279.1(2004):95-99.

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