Knowledge Management System of Institue of Mechanics, CAS
| Study on flow characteristics of solid/liquid system in lysozyme crystal growth | |
Cui HL(崔海亮) ; Yu Y(于泳); Chen WC(陈万春); Kang Q(康奇); Yu, Y (reprint author), Chinese Acad Sci, Inst Mech, Natl Micrograv Lab, Beijing 100080, Peoples R China.
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| Source Publication | Chinese Science Bulletin/中国科学通报(英文版)
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| 2007 | |
| Volume | 52Issue:9Pages:1196-1204 |
| ISSN | 1001-6538 |
| Abstract | During the process of lysozyme protein crystallization with batch method, the macroscopic flow field of solid/liquid system was observed by particle image velocimetry (PIV). Furthermore, a normal growth rate of (110) face and local flow field around a single protein crystal were obtained by a long work distance microscope. The experimental results showed that the average velocity, the maximal velocity of macroscopic solid/liquid system and the velocity of local flow field around single protein crystal were fluctuant. The effective boundary layer thickness delta(eff), the concentration at the interface Q and the characteristic velocity V were calculated using a convection-diffusion model. The results showed that the growth of lysozyme crystal in this experiment was dominated by interfacial kinetics rather than bulk transport, and the function of buoyancy-driven flow in bulk transport was small, however, the effect of bulk transport in crystal growth had a tendency to increase with the increase of lysozyme concentration. The calculated results, also showed that the order of magnitude of shear force was about 10(-21) N, which was much less than the bond force between the lysozyme molecules. Therefore the shear force induced by buoyancy-driven flows cannot remove the protein molecules from the interface of crystal. |
| Keyword | Protein Crystal Batch Method Buoyancy-driven Flows Particle Image Velocimetry Shear Force Protein Crystal Kinetics Crystallization Microgravity Convection Transport Virus |
| DOI | 10.1007/s11434-007-0176-2 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000247545800008 |
| WOS Keyword | PROTEIN CRYSTAL ; KINETICS ; CRYSTALLIZATION ; MICROGRAVITY ; CONVECTION ; TRANSPORT ; VIRUS |
| WOS Research Area | Science & Technology - Other Topics |
| WOS Subject | Multidisciplinary Sciences |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/33951 |
| Collection | 力学所知识产出(1956-2008) |
| Corresponding Author | Yu, Y (reprint author), Chinese Acad Sci, Inst Mech, Natl Micrograv Lab, Beijing 100080, Peoples R China. |
| Recommended Citation GB/T 7714 | Cui HL,Yu Y,Chen WC,et al. Study on flow characteristics of solid/liquid system in lysozyme crystal growth[J]. Chinese Science Bulletin/中国科学通报(英文版),2007,52,9,:1196-1204. |
| APA | 崔海亮,于泳,陈万春,康奇,&Yu, Y .(2007).Study on flow characteristics of solid/liquid system in lysozyme crystal growth.Chinese Science Bulletin/中国科学通报(英文版),52(9),1196-1204. |
| MLA | 崔海亮,et al."Study on flow characteristics of solid/liquid system in lysozyme crystal growth".Chinese Science Bulletin/中国科学通报(英文版) 52.9(2007):1196-1204. |
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