Quasi-3D Cytoskeletal Dynamics of Osteocytes under Fluid Flow

doi:10.1016/j.bpj.2010.08.064

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	Quasi-3D Cytoskeletal Dynamics of Osteocytes under Fluid Flow
	Baik AD; Lu XL; Qiu J; Huo B(霍波); Hillman EMC; Dong C; Guo XE; Huo B
发表期刊	Biophysical Journal
	2010
卷号	99 期号:9 页码:2812-2820
ISSN	0006-3495
摘要	Osteocytes respond to dynamic fluid shear loading by activating various biochemical pathways, mediating a dynamic process of bone formation and resorption. Whole-cell deformation and regional deformation of the cytoskeleton may be able to directly regulate this process. Attempts to image cellular deformation by conventional microscopy techniques have been hindered by low temporal or spatial resolution. In this study, we developed a quasi-three-dimensional microscopy technique that enabled us to simultaneously visualize an osteocyte's traditional bottom-view profile and a side-view profile at high temporal resolution. Quantitative analysis of the plasma membrane and either the intracellular actin or microtubule (MT) cytoskeletal networks provided characterization of their deformations over time. Although no volumetric dilatation of the whole cell was observed under flow, both the actin and MT networks experienced primarily tensile strains in all measured strain components. Regional heterogeneity in the strain field of normal strains was observed in the actin networks, especially in the leading edge to flow, but not in the MT networks. In contrast, side-view shear strains exhibited similar subcellular distribution patterns in both networks. Disruption of MT networks caused actin normal strains to decrease, whereas actin disruption had little effect on the MT network strains, highlighting the networks' mechanical interactions in osteocytes.
关键词	Living Endothelial-cells Viscoelastic Properties Articular-cartilage Shear-stress Bone-cells In-vitro Adhesion Deformation Prestress Strain
DOI	10.1016/j.bpj.2010.08.064
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000283912600015
关键词[WOS]	LIVING ENDOTHELIAL-CELLS ; VISCOELASTIC PROPERTIES ; ARTICULAR-CARTILAGE ; SHEAR-STRESS ; BONE-CELLS ; IN-VITRO ; ADHESION ; DEFORMATION ; PRESTRESS ; STRAIN
WOS研究方向	Biophysics
WOS类目	Biophysics
项目资助者	National Institutes of Health [AR058453, AR052417]
引用统计	被引频次：20[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/43623
专题	微重力重点实验室
通讯作者	Huo B
推荐引用方式 GB/T 7714	Baik AD,Lu XL,Qiu J,et al. Quasi-3D Cytoskeletal Dynamics of Osteocytes under Fluid Flow[J]. Biophysical Journal,2010,99,9,:2812-2820.
APA	Baik AD.,Lu XL.,Qiu J.,霍波.,Hillman EMC.,...&Huo B.(2010).Quasi-3D Cytoskeletal Dynamics of Osteocytes under Fluid Flow.Biophysical Journal,99(9),2812-2820.
MLA	Baik AD,et al."Quasi-3D Cytoskeletal Dynamics of Osteocytes under Fluid Flow".Biophysical Journal 99.9(2010):2812-2820.

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