| Detection of avian influenza virus subtype H5 using a biosensor based on imaging ellipsometry | |
Qi C(齐财); Tian XS ; Chen S(陈涉); Yan JH; Cao Z; Tian KG; Gao GF; Jin G(靳刚); Jin G
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| Source Publication | Biosensors & Bioelectronics
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| 2010 | |
| Volume | 25Issue:6Pages:1530-1534 |
| ISSN | 0956-5663 |
| Abstract | A novel method is reported for the detection of avian influenza virus subtype H5 using a biosensor based on high spatial resolution imaging ellipsometry (IE). Monoclonal antibodies specific to H5 hemagglutinin protein were immobilized on silicon wafers and used to capture virus particles. Resultant changes on the surface of the wafers were visualized directly in gray-scale on an imaging ellipsometry image. This preliminary study has shown that the assay is rapid and specific for the identification of avian influenza virus subtype H5. Compared with lateral-flow immunoassays, this biosensor not only has better sensitivity, but can also simultaneously perform multiplexed tests. These results suggest that this biosensor might be a valuable diagnostic toot for avian influenza virus detection. (c) 2009 Elsevier B.V. All rights reserved. |
| DOI | 10.1016/j.bios.2009.10.030 |
| URL | 查看原文 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000274868800045 |
| WOS Keyword | OFF-NULL ELLIPSOMETRY ; PROTEIN ; SURFACE ; IMMOBILIZATION ; INTERFACES ; BINDING |
| WOS Research Area | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
| WOS Subject | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology |
| Funding Organization | National Basic Research Program of China [2009CB320300] ; National High Technology Research and Development Program (863) of China [2006AA02Z4C2] ; National Basic Research Program (863) of China [2006AA02Z4C2] ; National Basic Research Program (Project 973) of China [2007CB310505] ; National Key Technologies RD Program [2006BAD06A01] ; Chinese Academy of Sciences [KJCX2-YW-Mo3, -M04] |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/43677 |
| Collection | 微重力重点实验室 |
| Corresponding Author | Jin G |
| Recommended Citation GB/T 7714 | Qi C,Tian XS,Chen S,et al. Detection of avian influenza virus subtype H5 using a biosensor based on imaging ellipsometry[J]. Biosensors & Bioelectronics,2010,25,6,:1530-1534. |
| APA | 齐财.,Tian XS.,陈涉.,Yan JH.,Cao Z.,...&Jin G.(2010).Detection of avian influenza virus subtype H5 using a biosensor based on imaging ellipsometry.Biosensors & Bioelectronics,25(6),1530-1534. |
| MLA | 齐财,et al."Detection of avian influenza virus subtype H5 using a biosensor based on imaging ellipsometry".Biosensors & Bioelectronics 25.6(2010):1530-1534. |
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