| Serum tumor marker detection on PEGylated lipid membrane using biosensor based on total internal reflection imaging ellipsometry | |
Zhang YB; Chen YY(陈艳艳) ; Jin G(靳刚); Chen, YY (reprint author), Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou 215125, Jiangsu, Peoples R China
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| Source Publication | Sensors and Actuators B-Chemical
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| 2011 | |
| Volume | 159Issue:1Pages:121-125 |
| ISSN | 0925-4005 |
| Abstract | We describe highly sensitive, label-free, real time detection of the biomarker CA19-9 using microarray biosensors based on total internal reflection imaging ellipsometry (TIRIE), in which anti-CA19-9 is immobilized onto the PEGylated phospholipid membrane. Strong resistance against non-specific adsorption of PEGylated lipid membrane allows direct serum assay without the blocking agent. We find that PEG density has an influence on antibody assembly and only a proper amount of PEG is appropriate for antigen binding. Two different methods for antibody assembly are compared and finally protein A is preferred due to its higher protein binding efficiency. The estimated detection limit of CA19-9 is 18.2 U/ml, which is lower than the cut-off value for normal level (37 U/ml). Regression analysis between the results of microarray biosensor and electrochemiluminescence immunoassay (ECLIA) suggests a high correlation (R = 0.989). The capability for real-time monitoring of tumor marker with high sensitivity and selectivity in clinically relevant samples opens up substantial possibilities for early cancer diagnosis and treatment. (C) 2011 Elsevier B.V. All rights reserved. |
| Keyword | Pegylated Phospholipid Membrane Total Internal Reflection Imaging Ellipsometry Ca19-9 Resectable Pancreatic Adenocarcinoma Perioperative Ca19-9 Levels (18)Fdg-pet Examination Predict Stage Protein Adsorption Immobilization Surfaces Antigen Cancer |
| Subject Area | Chemistry ; Electrochemistry ; Instruments & Instrumentation |
| DOI | 10.1016/j.snb.2011.06.059 |
| URL | 查看原文 |
| Indexed By | SCI ; EI |
| Language | 英语 |
| WOS ID | WOS:000295745900017 |
| WOS Keyword | RESECTABLE PANCREATIC ADENOCARCINOMA ; PERIOPERATIVE CA19-9 LEVELS ; (18)FDG-PET EXAMINATION ; PREDICT STAGE ; PROTEIN ; ADSORPTION ; IMMOBILIZATION ; SURFACES ; ANTIGEN ; CANCER |
| WOS Research Area | Chemistry ; Electrochemistry ; Instruments & Instrumentation |
| WOS Subject | Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation |
| Funding Organization | The authors would like to gratefully acknowledge financial supports from Ministry of Science and Technology of China (2008AA02Z419, 2009CB320302), Natural Science Foundation (10804083, 20845003) of China, Chinese Academy of Sciences (KJCX2-YW-M04, KJCX2-YW-M03) and Key Laboratory of Microgravity, Institute of Mechanics, CAS. |
| Department | NML纳米生物光学 |
| Classification | 二类/Q1 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/45066 |
| Collection | 微重力重点实验室 |
| Corresponding Author | Chen, YY (reprint author), Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou 215125, Jiangsu, Peoples R China |
| Recommended Citation GB/T 7714 | Zhang YB,Chen YY,Jin G,et al. Serum tumor marker detection on PEGylated lipid membrane using biosensor based on total internal reflection imaging ellipsometry[J]. Sensors and Actuators B-Chemical,2011,159,1,:121-125. |
| APA | Zhang YB,陈艳艳,靳刚,&Chen, YY .(2011).Serum tumor marker detection on PEGylated lipid membrane using biosensor based on total internal reflection imaging ellipsometry.Sensors and Actuators B-Chemical,159(1),121-125. |
| MLA | Zhang YB,et al."Serum tumor marker detection on PEGylated lipid membrane using biosensor based on total internal reflection imaging ellipsometry".Sensors and Actuators B-Chemical 159.1(2011):121-125. |
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