| Detection of alpha-fetoprotein through biological signal amplification by biosensor based on imaging ellipsometry | |
Huang CH; Chen YY(陈艳艳) ; Wang CX; Zhu W(朱伟); Ma HW; Jin G(靳刚); Chen, YY (reprint author), Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou, Peoples R China
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| Source Publication | Thin Solid Films
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| 2011 | |
| Volume | 519Issue:9Pages:2763-2767 |
| ISSN | 0040-6090 |
| Abstract | High sensitive and quantitative detection of alpha-fetoprotein (AFP) by biosensor based on imaging ellipsometry (BIE) through biological amplification was investigated. AFP firstly reacted with the rat monoclonal antibody (rat-mAb) initially immobilized on glutaraldehyde modified silicon surface, then rabbit anti-human AFP polyclonal antibodies (Rabbit-pAb) and goat anti-rabbit IgG (goat-IgG) were sequentially applied to amplify signal. Results revealed that signal was enhanced approximately six fold. The linear range of AFP detection was 20.0-200.0 ng/ml with a low limit of 5.0 ng/ml (S/N = 3). The cross-reaction rate was less than 5.2% evaluated by biomarker (carcinoembryonic antigen, carbohydrate 19-9 and carbohydrate antigen 242) and two common proteins (human serum albumin, fibrinogen) and their mixture. Coefficient variation (CV) for intra-slide and inter-slide reproducibility were 10.3%, 6.6%, 6.3% and 10.7%, 7.9%, 6.4% for 41.6 ng/ml, 83.2 ng/ml and 128.4 ng/ml AFP. In clinic application, cut-off value at 28.6 ng/ml was also determined with sensitivity 0.72 and specificity 0.94. Results of 47 clinic patient samples detected by BIE were in good agreement with those of electrochemiluminescence immunoassay (ECLIA) (R2 = 0.9949). The area under receiver operating characteristic curve (ROC) curve is 0.89. Statistical analysis showed that BIE is high agreement with ECLIA (Kappa = 0.733, U = 3.21>U0.01). It shows a potential for hepatocellular carcinoma (HCC) diagnosis. (C) 2010 Elsevier B.V. All rights reserved. |
| Keyword | Imaging Ellipsometry Biosensor Signal Amplification Alpha-fetoprotein Hepatocellular Carcinoma Protein Microarrays Visualization Prospects Cancer Serum |
| Subject Area | Materials Science ; Physics |
| DOI | 10.1016/j.tsf.2010.11.064 |
| URL | 查看原文 |
| Indexed By | SCI ; EI |
| Language | 英语 |
| WOS ID | WOS:000289174200042 |
| WOS Keyword | PROTEIN MICROARRAYS ; VISUALIZATION ; PROSPECTS ; CANCER ; SERUM |
| WOS Research Area | Materials Science ; Physics |
| WOS Subject | Materials Science, Multidisciplinary ; Materials Science, Coatings & Films ; Physics, Applied ; Physics, Condensed Matter |
| Department | NML纳米生物光学 |
| Classification | 二类/Q2 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/45101 |
| Collection | 微重力重点实验室 |
| Corresponding Author | Chen, YY (reprint author), Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou, Peoples R China |
| Recommended Citation GB/T 7714 | Huang CH,Chen YY,Wang CX,et al. Detection of alpha-fetoprotein through biological signal amplification by biosensor based on imaging ellipsometry[J]. Thin Solid Films,2011,519,9,:2763-2767. |
| APA | Huang CH.,陈艳艳.,Wang CX.,朱伟.,Ma HW.,...&Chen, YY .(2011).Detection of alpha-fetoprotein through biological signal amplification by biosensor based on imaging ellipsometry.Thin Solid Films,519(9),2763-2767. |
| MLA | Huang CH,et al."Detection of alpha-fetoprotein through biological signal amplification by biosensor based on imaging ellipsometry".Thin Solid Films 519.9(2011):2763-2767. |
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