Gradual Immobilization Processes of Molecules during Transitions from Solute to Solid States

doi:10.1021/cg100869r

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	Gradual Immobilization Processes of Molecules during Transitions from Solute to Solid States
	Dai GL(戴国亮); Sazaki Gen; Matsui Takuro; Tsukamoto Katsuo; Nakajima Kazuo; Kang Q(康琦); Hu WR(胡文瑞); Sazaki, G (reprint author), Hokkaido Univ, Inst Low Temp Sci, N19-W8 Kita Ku, Sapporo, Hokkaido 0600819, Japan
发表期刊	Crystal Growth & Design
	2011
卷号	11 期号:1 页码:88-92
ISSN	1528-7483
摘要	The adsorption/desorption behavior of mobile solute molecules at a solution-crystal interface has been explored using crystals of model protein hen egg-white lysozyme (HEWL) and fluorescent-labeled HEWL (F-HEWL) molecules. We have tracked the transient processes occurring during adsorption/desorption of identical F-HEWL molecules on a tetragonal HEWL crystal surface by single-molecule visualization using a total internal reflection fluorescent microscope and pulsed discontinuous laser illumination. We found an induction period (similar to 70 min) after which the number density of F-HEWL molecules adsorbed mainly on steps increased linearly with the adsorption time. We show direct evidence that the residence time of molecules on the crystal surface gradually increases during the transition process from a solute species to the crystal after successive multistep processes.
关键词	Reflection Fluorescence Microscopy Single-protein Molecules Crystals Adsorption Diffusion Interface Surface
学科领域	Chemistry ; Crystallography ; Materials Science
DOI	10.1021/cg100869r
URL	查看原文
收录类别	SCI ; EI
语种	英语
WOS记录号	WOS:000285735100015
关键词[WOS]	REFLECTION FLUORESCENCE MICROSCOPY ; SINGLE-PROTEIN MOLECULES ; CRYSTALS ; ADSORPTION ; DIFFUSION ; INTERFACE ; SURFACE
WOS研究方向	Chemistry ; Crystallography ; Materials Science
WOS类目	Chemistry, Multidisciplinary ; Crystallography ; Materials Science, Multidisciplinary
项目资助者	The authors thank H. Higuchi (The University of Tokyo) for valuable discussions, and they acknowledge grants from the National Basic Research Program of China (973 Program) (G.D.: No. 2011CB710901), the Knowledge Innovation Program of the Chinese Academy of Sciences (G.D.: No. KJCX2-YW-L08), and the National Nature Science Foundation of China (G.D.: No. 20603043), and Grants-in-Aid (G.S.: Nos. 17034007 and 18360003) from the Scientific Research of the Ministry of Education, Science and Culture, Japan. One of the authors (G.D.) acknowledges a grant from Tohoku University as a visiting professor. This work was carried out as a part of "Project Research B" at the Center for Interdisciplinary Research, Tohoku University, as well as the "Ground-based Research Announcement for Space Utilization" of the Japan Space Forum.
课题组名称	NML复杂流体
论文分区	一类
引用统计	被引频次：10[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/45110
专题	微重力重点实验室
通讯作者	Sazaki, G (reprint author), Hokkaido Univ, Inst Low Temp Sci, N19-W8 Kita Ku, Sapporo, Hokkaido 0600819, Japan
推荐引用方式 GB/T 7714	Dai GL,Sazaki Gen,Matsui Takuro,et al. Gradual Immobilization Processes of Molecules during Transitions from Solute to Solid States[J]. Crystal Growth & Design,2011,11,1,:88-92.
APA	戴国亮.,Sazaki Gen.,Matsui Takuro.,Tsukamoto Katsuo.,Nakajima Kazuo.,...&Sazaki, G .(2011).Gradual Immobilization Processes of Molecules during Transitions from Solute to Solid States.Crystal Growth & Design,11(1),88-92.
MLA	戴国亮,et al."Gradual Immobilization Processes of Molecules during Transitions from Solute to Solid States".Crystal Growth & Design 11.1(2011):88-92.

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