| Gradual Immobilization Processes of Molecules during Transitions from Solute to Solid States | |
Dai GL(戴国亮) ; Sazaki Gen; Matsui Takuro; Tsukamoto Katsuo; Nakajima Kazuo; Kang Q(康琦); Hu WR(胡文瑞); Sazaki, G (reprint author), Hokkaido Univ, Inst Low Temp Sci, N19-W8 Kita Ku, Sapporo, Hokkaido 0600819, Japan
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| Source Publication | Crystal Growth & Design
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| 2011 | |
| Volume | 11Issue:1Pages:88-92 |
| ISSN | 1528-7483 |
| Abstract | The adsorption/desorption behavior of mobile solute molecules at a solution-crystal interface has been explored using crystals of model protein hen egg-white lysozyme (HEWL) and fluorescent-labeled HEWL (F-HEWL) molecules. We have tracked the transient processes occurring during adsorption/desorption of identical F-HEWL molecules on a tetragonal HEWL crystal surface by single-molecule visualization using a total internal reflection fluorescent microscope and pulsed discontinuous laser illumination. We found an induction period (similar to 70 min) after which the number density of F-HEWL molecules adsorbed mainly on steps increased linearly with the adsorption time. We show direct evidence that the residence time of molecules on the crystal surface gradually increases during the transition process from a solute species to the crystal after successive multistep processes. |
| Keyword | Reflection Fluorescence Microscopy Single-protein Molecules Crystals Adsorption Diffusion Interface Surface |
| Subject Area | Chemistry ; Crystallography ; Materials Science |
| DOI | 10.1021/cg100869r |
| URL | 查看原文 |
| Indexed By | SCI ; EI |
| Language | 英语 |
| WOS ID | WOS:000285735100015 |
| WOS Keyword | REFLECTION FLUORESCENCE MICROSCOPY ; SINGLE-PROTEIN MOLECULES ; CRYSTALS ; ADSORPTION ; DIFFUSION ; INTERFACE ; SURFACE |
| WOS Research Area | Chemistry ; Crystallography ; Materials Science |
| WOS Subject | Chemistry, Multidisciplinary ; Crystallography ; Materials Science, Multidisciplinary |
| Funding Organization | The authors thank H. Higuchi (The University of Tokyo) for valuable discussions, and they acknowledge grants from the National Basic Research Program of China (973 Program) (G.D.: No. 2011CB710901), the Knowledge Innovation Program of the Chinese Academy of Sciences (G.D.: No. KJCX2-YW-L08), and the National Nature Science Foundation of China (G.D.: No. 20603043), and Grants-in-Aid (G.S.: Nos. 17034007 and 18360003) from the Scientific Research of the Ministry of Education, Science and Culture, Japan. One of the authors (G.D.) acknowledges a grant from Tohoku University as a visiting professor. This work was carried out as a part of "Project Research B" at the Center for Interdisciplinary Research, Tohoku University, as well as the "Ground-based Research Announcement for Space Utilization" of the Japan Space Forum. |
| Department | NML复杂流体 |
| Classification | 一类 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/45110 |
| Collection | 微重力重点实验室 |
| Corresponding Author | Sazaki, G (reprint author), Hokkaido Univ, Inst Low Temp Sci, N19-W8 Kita Ku, Sapporo, Hokkaido 0600819, Japan |
| Recommended Citation GB/T 7714 | Dai GL,Sazaki Gen,Matsui Takuro,et al. Gradual Immobilization Processes of Molecules during Transitions from Solute to Solid States[J]. Crystal Growth & Design,2011,11,1,:88-92. |
| APA | 戴国亮.,Sazaki Gen.,Matsui Takuro.,Tsukamoto Katsuo.,Nakajima Kazuo.,...&Sazaki, G .(2011).Gradual Immobilization Processes of Molecules during Transitions from Solute to Solid States.Crystal Growth & Design,11(1),88-92. |
| MLA | 戴国亮,et al."Gradual Immobilization Processes of Molecules during Transitions from Solute to Solid States".Crystal Growth & Design 11.1(2011):88-92. |
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