| Approach to Quantitative Detection of CD146 with the Label-free Protein Biosensor Based on Imaging Ellipsometry | |
Niu Y(牛宇) ; Liu L(刘丽); Yan XY; Jin G(靳刚); Jin, G (reprint author), Chinese Acad Sci, NML, Inst Mech, 15 Bei Si Huan W Rd, Beijing 100190, Peoples R China
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| Source Publication | SENSORS, CAMERAS, AND SYSTEMS FOR INDUSTRIAL, SCIENTIFIC, AND CONSUMER APPLICATIONS XII |
| 2011 | |
| Pages | 78750R |
| Conference Name | Conference on Sensors, Cameras, and Systems for Industrial, Scientific, and Consumer Applications XII |
| Conference Date | JAN 25-27, 2011 |
| Conference Place | San Francisco, CA |
| Abstract | CD146 glycoprotein belonging to cell adhesion molecules is considered to be a novel target on endothelial cell involved in tumor angiogenesis. The biosensor based on imaging ellipsometry (BIE) which is performed in null and off-null mode is used for CD146 detection as a trial by the following steps. Firstly, anti-CD146 antibody as ligand is immobilized on Protein G modified silicon substrate. Then, CD146 test is carried out and its calibration curve is established for the requirement of quantitative detection. Finally, 18 serum samples are detected quantitatively and their results are validated by ELISA's. The sensitivity for CD146 detection achieves the order of ng/ml and the relationship between BIE signal y (grayscale value) and CD146 concentration x (ng/ml) is y=3.3ln(x) +91.3. Compared with ELISA's, the majority of results are in agreement, and the results of two approaches have significant statistic relevance. |
| Keyword | Cd146 Detection Biosensor Imaging Ellipsometry Mel-cam Antibody Visualization Growth Cancer |
| WOS ID | WOS:000299998500249 |
| Department | NML纳米生物光学 |
| ISBN | 978-0-8194-8412-3 |
| URL | 查看原文 |
| Indexed By | CPCI-S |
| Language | 英语 |
| Citation statistics | |
| Document Type | 会议论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/45315 |
| Collection | 微重力重点实验室 |
| Corresponding Author | Jin, G (reprint author), Chinese Acad Sci, NML, Inst Mech, 15 Bei Si Huan W Rd, Beijing 100190, Peoples R China |
| Recommended Citation GB/T 7714 | Niu Y,Liu L,Yan XY,et al. Approach to Quantitative Detection of CD146 with the Label-free Protein Biosensor Based on Imaging Ellipsometry[C]SENSORS, CAMERAS, AND SYSTEMS FOR INDUSTRIAL, SCIENTIFIC, AND CONSUMER APPLICATIONS XII,2011:78750R. |
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| File Name/Size | DocType | Version | Access | License | ||
| Conf2011-086.pdf（289KB） | 会议论文 | 开放获取 | CC BY-NC-SA | View Download | ||
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