| Probe stiffness regulates receptor-ligand bond lifetime under force | |
Zhang Y(章燕) ; Lv SQ(吕守芹); Long M(龙勉); Long, MA (reprint author), Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Beijing 100190, Peoples R China
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| Source Publication | SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY |
| 2011 | |
| Pages | 923-929 |
| Conference Name | Symposium on Multiscale Modeling and Simulation of Materials/11th International Conference in Asia of the International-Union-of-Materials-Research-Societies |
| Conference Date | SEP 25-28, 2010 |
| Conference Place | Qingdao, PEOPLES R CHINA |
| Abstract | Receptor-ligand bond dissociation under applied force is crucial to elucidate its biological functionality when the molecular bond is usually connected to a mechanical probe. While the impact of probe stiffness, k, on bond rupture force has recently attracted more and more attention, the mechanism of how it affects the bond lifetime, however, remains unclear. Here we quantified the dissociation lifetime of selectin-ligand bond using an optical trap assay with low stiffness ranging from 3.5x10(-3) to 4.7x10(-2) pN/nm. Our results indicated that bond lifetime yielded distinct distributions with different probe stiffness, implying the stochastic feature of bond dissociation. It was also found that the mean lifetime varied with probe stiffness and that the catch bond nature was visualized at k >= 3.0x10(-2) pN/nm. This work furthered the understanding of the forced dissociation of selectin-ligand bond at varied probe stiffness, which is physiologically relevant to the tethered rolling of leukocytes under blood flow. |
| Keyword | Probe Stiffness Bond Lifetime Optical Trap |
| Department | 中科院国家微重力实验室 |
| URL | 查看原文 |
| Indexed By | CPCI（ISTP） |
| Language | 英语 |
| Document Type | 会议论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/45325 |
| Collection | 微重力重点实验室 |
| Corresponding Author | Long, MA (reprint author), Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Beijing 100190, Peoples R China |
| Recommended Citation GB/T 7714 | Zhang Y,Lv SQ,Long M,et al. Probe stiffness regulates receptor-ligand bond lifetime under force[C]SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY,2011:923-929. |
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