| Fluid flow-induced calcium response in early or late differentiated osteoclasts | |
Li P(李平) ; Hu M(胡漫); Sun SJ(孙树津); Zhang Y(章燕); Gao YX(高宇欣); Long M(龙勉); Huo B(霍波); Zhang D; Huo, B; Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Key Lab Micrograv, 15 N 4th Ring Rd, Beijing 100190, Peoples R China.
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| Source Publication | ANNALS OF BIOMEDICAL ENGINEERING
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| 2012-09-01 | |
| Volume | 40Issue:9Pages:1874-1883 |
| ISSN | 0090-6964 |
| Abstract | Intracellular calcium oscillation caused by receptor activator of nuclear factor kappa-B ligand has been demonstrated to promote the differentiation of osteoclasts. Osteoclasts are recruited on the surface of trabeculae, and are exposed to fluid flow caused by the deformation of the bone matrix. However, the roles of fluid shear stress (FSS) on calcium response during the differentiation process of osteoclasts are still unknown. In the current study, the formation of tartrate-resistant acid phosphatase-positive, multinucleated osteoclasts from RAW264.7 macrophage cells were induced by co-culturing them with the conditioned medium from MC3T3-E1 osteoblasts. The in situ observations showed a high correlation between the area and the nuclear number of osteoclasts. The cells were stimulated by FSS at different levels (1 or 10 dyne/cm(2)) before (0 day) or after being induced for 4 or 8 days. The mechanically-induced calcium response was recorded and analyzed. The results indicated a different property of calcium oscillation for the osteoclasts in different fusion stages (i.e., more calcium-responsive peaks appeared in small osteoclasts than those in the larger ones). The rates of calcium influx decreased and the time of recovery in osteoclast cytosol increased along with the fusion of osteoclasts. In addition, increasing the FSS level enhanced the calcium oscillation of osteoclasts at early induction (4 days). However, this effect was weakened at the late induction (8 days). The present work could help provide understanding regarding the mechanism of the involvement of calcium in mechanically induced bone remodeling. |
| Keyword | Osteoclasts Fluid Shear Stress Calcium Oscillation Induction Time Differentiation Mechanical Strain Rat Osteoclasts Bone-resorption Activation Expression Cells Ca2++ |
| Subject Area | 生物力学 |
| URL | 查看原文 |
| Indexed By | SCI ; EI |
| Language | 英语 |
| WOS ID | WOS:000307400900003 |
| Funding Organization | This work was supported by the National Natural Science Foundation of China [30970707 (BH) and 31070829 (DZ)], the National Key Basic Research Foundation of China (2011CB710904), and the Knowledge Innovation Project of the CAS (KJCX2-YW-L08) (ML). |
| Department | NML分子-细胞生物力学与空间生命科学 |
| Classification | 二类/Q2 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/46584 |
| Collection | 微重力重点实验室 |
| Corresponding Author | Huo, B; Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Key Lab Micrograv, 15 N 4th Ring Rd, Beijing 100190, Peoples R China. |
| Recommended Citation GB/T 7714 | Li P,Hu M,Sun SJ,et al. Fluid flow-induced calcium response in early or late differentiated osteoclasts[J]. ANNALS OF BIOMEDICAL ENGINEERING,2012,40,9,:1874-1883. |
| APA | Li P.,Hu M.,Sun SJ.,Zhang Y.,Gao YX.,...&Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Key Lab Micrograv, 15 N 4th Ring Rd, Beijing 100190, Peoples R China..(2012).Fluid flow-induced calcium response in early or late differentiated osteoclasts.ANNALS OF BIOMEDICAL ENGINEERING,40(9),1874-1883. |
| MLA | Li P,et al."Fluid flow-induced calcium response in early or late differentiated osteoclasts".ANNALS OF BIOMEDICAL ENGINEERING 40.9(2012):1874-1883. |
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