| Double spiral microchannel for label-free tumor cell separation and enrichment |
| Sun JS ; Li MM; Liu C(刘超); Zhang Y; Liu DB; Liu WW; Hu GQ(胡国庆); Jiang XY; Sun, JS (reprint author), Natl Ctr NanoSci & Technol, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China.
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| Source Publication | LAB ON A CHIP
(IF:6.914[JCR-2018],6.575[5-Year]) |
| 2012-06
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| Volume | 12Issue:20Pages:3952-3960 |
| ISSN | 1473-0197
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| Abstract | This work reports on a passive double spiral microfluidic device allowing rapid and label-free tumor cell separation and enrichment from diluted peripheral whole blood, by exploiting the size-dependent hydrodynamic forces. A numerical model is developed to simulate the Dean flow inside the curved geometry and to track the particle/cell trajectories, which is validated against the experimental observations and serves as a theoretical foundation for optimizing the operating conditions. Results from separating tumor cells (MCF-7 and Hela) spiked into whole blood indicate that 92.28% of blood cells and 96.77% of tumor cells are collected at the inner and the middle outlet, respectively, with 88.5% tumor recovery rate at a throughput of 3.33 x 10(7) cells min(-1). We expect that this label-free microfluidic platform, driven by purely hydrodynamic forces, would have an impact on fundamental and clinical studies of circulating tumor cells. |
| Subject Area | 微机电系统中的流体力学问题
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| DOI | 10.1039/c2lc40679a
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| URL | 查看原文
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| Indexed By | SCI
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| Language | 英语
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| WOS ID | WOS:000308894600016
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| WOS Keyword | METASTATIC BREAST-CANCER; PERIPHERAL-BLOOD; DIELECTROPHORETIC SEPARATION; MICROFLUIDIC DEVICES; CARCINOMA-CELLS; PARTICLES; CHIP; SIZE; FLOW; TECHNOLOGY
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| Funding Organization | MOST [2011CB933201, 2009CB930001, 2011CB707604]; NSFC [51105086, 21025520, 90813032, 50890182]; CAS [KJCX2-YW-M15]
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| Department | LNM微纳米流体力学
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| Classification | 一类
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| Citation statistics |
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| Document Type | 期刊论文
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| Identifier | http://dspace.imech.ac.cn/handle/311007/47111
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| Collection | 非线性力学国家重点实验室
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| Corresponding Author | Sun, JS (reprint author), Natl Ctr NanoSci & Technol, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China. |
Recommended Citation
GB/T 7714 |
Sun JS,Li MM,Liu C,et al. Double spiral microchannel for label-free tumor cell separation and enrichment[J]. LAB ON A CHIP,2012,12,20,:3952-3960.
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| APA |
Sun JS.,Li MM.,Liu C.,Zhang Y.,Liu DB.,...&Sun, JS .(2012).Double spiral microchannel for label-free tumor cell separation and enrichment.LAB ON A CHIP,12(20),3952-3960.
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| MLA |
Sun JS,et al."Double spiral microchannel for label-free tumor cell separation and enrichment".LAB ON A CHIP 12.20(2012):3952-3960.
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