| Two dimensional barcode-inspired automatic analysis for arrayed microfluidic immunoassays | |
Zhang Y; Qiao LB; Ren YK; Wang XW(王绪伟) ; Gao M; Tang YF; Xi JJ; Fu TM; Jiang XY; Fu, TM (reprint author), Peking Univ, Coll Engn, Beijing 100871, Peoples R China.
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| Source Publication | BIOMICROFLUIDICS
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| 2013-05-09 | |
| Volume | 7Issue:3Pages:034110/1-034110/12 |
| ISSN | 1932-1058 |
| Abstract | The usability of many high-throughput lab-on-a-chip devices in point-of-care applications is currently limited by the manual data acquisition and analysis process, which are labor intensive and time consuming. Based on our original design in the biochemical reactions, we proposed here a universal approach to perform automatic, fast, and robust analysis for high-throughput array-based microfluidic immunoassays. Inspired by two-dimensional (2D) barcodes, we incorporated asymmetric function patterns into a microfluidic array. These function patterns provide quantitative information on the characteristic dimensions of the microfluidic array, as well as mark its orientation and origin of coordinates. We used a computer program to perform automatic analysis for a high-throughput antigen/antibody interaction experiment in 10 s, which was more than 500 times faster than conventional manual processing. Our method is broadly applicable to many other microchannel-based immunoassays. |
| Keyword | Bar Codes Two Dimensional Antigen/antibody Interactions Automatic Analysis Biochemical Reactions Lab-on-a-chip Devices Microfluidic Array Microfluidic Immunoassay Quantitative Information Universal Approach |
| Subject Area | 交叉与边缘领域的力学 |
| URL | 查看原文 |
| Indexed By | SCI ; EI |
| Language | 英语 |
| WOS ID | WOS:000321145800011 |
| Funding Organization | National Natural Science Foundation of China [21025520]; Chinese Academy of Sciences [KJCX2-YW-M15]; Ministry of Science and Technology of the People's Republic of China [2012AA030608] |
| Classification | 一类 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/47358 |
| Collection | 非线性力学国家重点实验室 |
| Corresponding Author | Fu, TM (reprint author), Peking Univ, Coll Engn, Beijing 100871, Peoples R China. |
| Recommended Citation GB/T 7714 | Zhang Y,Qiao LB,Ren YK,et al. Two dimensional barcode-inspired automatic analysis for arrayed microfluidic immunoassays[J]. BIOMICROFLUIDICS,2013,7,3,:034110/1-034110/12. |
| APA | Zhang Y.,Qiao LB.,Ren YK.,Wang XW.,Gao M.,...&Fu, TM .(2013).Two dimensional barcode-inspired automatic analysis for arrayed microfluidic immunoassays.BIOMICROFLUIDICS,7(3),034110/1-034110/12. |
| MLA | Zhang Y,et al."Two dimensional barcode-inspired automatic analysis for arrayed microfluidic immunoassays".BIOMICROFLUIDICS 7.3(2013):034110/1-034110/12. |
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