| Rapid and label-free detection of porcine reproductive and respiratory syndrome virus on nanoscale by biosensor based on imaging ellipsometry | |
Huang CH; Hou CJ; Huo DQ; Chen YY ; Jin G(靳刚); Huo, DQ (reprint author), Chongqing Univ, Bioengn Coll, Minist Educ, Key Lab Biorheol Sci & Technol, Chongqing 400030, Peoples R China.
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| Source Publication | INTEGRATED FERROELECTRICS
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| 2013 | |
| Volume | 145Issue:1Pages:122-129 |
| ISSN | 1058-4587 |
| Abstract | This study describes a simple and rapid method for virus detection on nanoscale measurement utilizing biosensor based on imaging ellipsometry. Porcine reproductive and respiratory syndrome virus (PRRSV) was served as an experimental model for demonstration. Staphylococcal protein A was firstly attached onto carboxyl-modified silicon substrate and purified anti-PRRSV IgG obtained from hyperimmune sera combined with Protein A for oriented immobilization to form capture layer. PRRSV in samples can then be specifically grabbed onto the capture layer. The signal (grayscale value) after virus combination is intuitively quantified by imaging ellipsometry. The minimum detection limit was as low as 2.4 x 10(3) median cell infectious dose (CCID50 mL(-1)) infectious virus. Application the proposed method to 20 clinic samples demonstrated that the result was significant correlated (R = 0.85) with virus isolation. The detection time was only 20min expended. |
| Keyword | Biosensor Imaging Ellipsometry Prrsv |
| Subject Area | 交叉与边缘领域的力学 |
| URL | 查看原文 |
| Indexed By | SCI ; EI |
| Language | 英语 |
| WOS ID | WOS:000321687200016 |
| Funding Organization | laboratory of Sichuan, China [NJ2009-04] |
| Department | NML纳米生物光学 |
| Classification | Q4 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/47361 |
| Collection | 微重力重点实验室 |
| Corresponding Author | Huo, DQ (reprint author), Chongqing Univ, Bioengn Coll, Minist Educ, Key Lab Biorheol Sci & Technol, Chongqing 400030, Peoples R China. |
| Recommended Citation GB/T 7714 | Huang CH,Hou CJ,Huo DQ,et al. Rapid and label-free detection of porcine reproductive and respiratory syndrome virus on nanoscale by biosensor based on imaging ellipsometry[J]. INTEGRATED FERROELECTRICS,2013,145,1,:122-129. |
| APA | Huang CH,Hou CJ,Huo DQ,Chen YY,Jin G,&Huo, DQ .(2013).Rapid and label-free detection of porcine reproductive and respiratory syndrome virus on nanoscale by biosensor based on imaging ellipsometry.INTEGRATED FERROELECTRICS,145(1),122-129. |
| MLA | Huang CH,et al."Rapid and label-free detection of porcine reproductive and respiratory syndrome virus on nanoscale by biosensor based on imaging ellipsometry".INTEGRATED FERROELECTRICS 145.1(2013):122-129. |
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