| Spreading area and shape regulate apoptosis and differentiation of osteoblasts | |
Fu RR(付瑞荣) ; Liu QL(刘琴丽); Song GB; Baik A; Hu M(胡漫); Sun SJ(孙树津); Guo XE; Long M(龙勉); Huo B(霍波); Long, M (reprint author), Chinese Acad Sci, Key Lab Micrograv, Inst Mech, Beijing 100190, Peoples R China.
| |
| Source Publication | BIOMEDICAL MATERIALS
 |
| 2013-10 | |
| Volume | 8Issue:5Pages:055005/1-055005/8 |
| ISSN | 1748-6041 |
| Abstract | The in vivo observations have indicated that at the remodeling sites of bone, the spreading area or shape of preosteoblasts is confined by the mineralized matrix. But it remains unknown whether this spreading confinement regulates the differentiation or apoptosis of osteoblasts. In the present study, osteoblast-like cells (MC3T3-E1) were seeded on micropatterned islands with different area and shape. The expression of three osteogenic differentiation markers was measured by immunofluorescence staining and apoptotic cells were detected using a terminal deoxyribonucleotidyl transferase-mediated dUTP nick end labelling assay kit. The membrane fluorescence staining results showed that the actual spreading area of micropatterned osteoblasts coincided with the designed value. When the area of a micropatterned cell was confined as 314 or 615 mu m(2), which was lower than that of freely spreading osteoblasts, the circular shape promoted the expression of osteogenic differentiation markers and the percentage of apoptotic osteoblasts compared with the branched shape. This shape-regulated differentiation and apoptosis of osteoblasts with confined spreading area were abolished when actin polymerization was inhibited by cytochalasin D. The present study gives an insight into the roles of spreading morphology on osteoblastic differentiation and apoptosis. |
| Subject Area | 生物力学 |
| URL | 查看原文 |
| Indexed By | SCI ; EI |
| Language | 英语 |
| WOS ID | WOS:000324922200005 |
| Funding Organization | National Natural Science Foundation of China [30970707, 31110103918]; National Key Basic Research Foundation of China [2011CB710904] |
| Department | NML分子-细胞生物力学与空间生命科学 |
| Classification | 二类 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/47499 |
| Collection | 微重力重点实验室 |
| Corresponding Author | Long, M (reprint author), Chinese Acad Sci, Key Lab Micrograv, Inst Mech, Beijing 100190, Peoples R China. |
| Recommended Citation GB/T 7714 | Fu RR,Liu QL,Song GB,et al. Spreading area and shape regulate apoptosis and differentiation of osteoblasts[J]. BIOMEDICAL MATERIALS,2013,8,5,:055005/1-055005/8. |
| APA | 付瑞荣.,刘琴丽.,Song GB.,Baik A.,胡漫.,...&Long, M .(2013).Spreading area and shape regulate apoptosis and differentiation of osteoblasts.BIOMEDICAL MATERIALS,8(5),055005/1-055005/8. |
| MLA | 付瑞荣,et al."Spreading area and shape regulate apoptosis and differentiation of osteoblasts".BIOMEDICAL MATERIALS 8.5(2013):055005/1-055005/8. |
| Files in This Item: | Download All | |||||
| File Name/Size | DocType | Version | Access | License | ||
| IMCAS-J2013-278.pdf（941KB） | 开放获取 | -- | View Download | |||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment