| Vesicle adhesion visualized with total internal reflection imaging ellipsometry biosensor | |
Liu L(刘丽) ; Viallat A; Jin G(靳刚); Viallat, A (reprint author), Aix Marseille Univ, CNRS, UMR 7333, INSERM,LAI,UMR 1067, F-13009 Marseille, France.
| |
| Source Publication | SENSORS AND ACTUATORS B-CHEMICAL
 |
| 2014-01 | |
| Volume | 190Pages:221-226 |
| ISSN | 0925-4005 |
| Abstract | The behavior of giant lipid vesicles (GUVs) interacting with an adhesive surface coated with poly-L-lysine is investigated by a biosensor based on total internal reflection imaging ellipsometry (TIRIE). The adhesion of GUVs on the adhesive surface is regulated by the concentration of the poly-L-lysine coating, the pH of the vesicle suspension, and the flow rate of the suspending fluid. The TIRIE biosensor detects very clearly and sensitively GUV adsorption, flattening, rupture, and de-adhesion, all these behaviors being independently observed by phase contrast microscopy. These results show that the setting of the TIRIE biosensor, optimized for molecular sensing can be extended to GUV sensing. This work paves the way to develop a new sensitive high-throughput, low-consumption and low-cost biosensor technology for micron-size objects such as cells, capsules and liposomes. |
| Keyword | Giant Lipid Vesicles (Guvs) Vesicles Adhesion Non-specific Adhesion Total Internal Reflection Imaging Ellipsometry (Tirie) Biosensor |
| Subject Area | 交叉与边缘领域的力学 |
| URL | 查看原文 |
| Indexed By | SCI ; EI |
| Language | 英语 |
| WOS ID | WOS:000326687700029 |
| Department | NML纳米生物光学 |
| Classification | 二类/Q1 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/48076 |
| Collection | 微重力重点实验室 |
| Corresponding Author | Viallat, A (reprint author), Aix Marseille Univ, CNRS, UMR 7333, INSERM,LAI,UMR 1067, F-13009 Marseille, France. |
| Recommended Citation GB/T 7714 | Liu L,Viallat A,Jin G,et al. Vesicle adhesion visualized with total internal reflection imaging ellipsometry biosensor[J]. SENSORS AND ACTUATORS B-CHEMICAL,2014,190:221-226. |
| APA | 刘丽,Viallat A,靳刚,&Viallat, A .(2014).Vesicle adhesion visualized with total internal reflection imaging ellipsometry biosensor.SENSORS AND ACTUATORS B-CHEMICAL,190,221-226. |
| MLA | 刘丽,et al."Vesicle adhesion visualized with total internal reflection imaging ellipsometry biosensor".SENSORS AND ACTUATORS B-CHEMICAL 190(2014):221-226. |
| Files in This Item: | Download All | |||||
| File Name/Size | DocType | Version | Access | License | ||
| IMCAS-J2014-003.pdf（1633KB） | 开放获取 | -- | View Download | |||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment