Droplet-in-oil array for picoliter-scale analysis based on sequential inkjet printing

doi:10.1039/c5lc00356c

IMECH-IR > 非线性力学国家重点实验室

	Droplet-in-oil array for picoliter-scale analysis based on sequential inkjet printing
	Sun YN; Chen XD(陈晓东); Zhou XG; Zhu JB; Yu YD; Yu, YD (reprint author), Chinese Acad Sci, Inst Semicond, State Key Lab Integrated Optoelect, POB 912, Beijing 100083, Peoples R China.
发表期刊	LAB ON A CHIP
	2015
卷号	15 期号:11 页码:2429-2436
ISSN	1473-0197
摘要	In recent years, inkjet printing, as a new method to fabricate microdroplet microarrays, has been increasingly applied in the field of biochemical diagnostics. To further improve the general applicability of the inkjet printing technology in fabricating biochemical chips, in this work, we introduce a model to describe the multiple injection procedure implemented by the inkjet printing approach, with experimental verification. The multiple injection model demonstrates a new sequential inkjet printing method that generates picoliter-scale multicomponent droplet-in-oil arrays via multistep printing on uniform planar substrates. Based on our previous work on double-inkjet printing, this technique adapts the piezoelectric inkjet printing technology to fabricate an oil droplet array, into which multiple precise injections of secondary droplets with different compositions and volumes can be automatically printed in the required sequence, simultaneously addressing the evaporation issues associated with printing picoliter droplets without external assistance. In this paper, we first describe the theory and characterize the model, which account for the basic principles of sequential inkjet printing, as well as validate the design in terms of multiple injections, droplet fusion, and rapid mixing. The feasibility and effectiveness of the method are also demonstrated in a dual fluorescence assay and a beta-galactosidase enzyme inhibition assay. We believe that applying the sequential inkjet printing methodology in existing inkjet printing devices will enhance their use as universal diagnostic tools as well as accelerate the adoption of inkjet printing in multistep screening experiments.
DOI	10.1039/c5lc00356c
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000354963400010
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics
WOS类目	Biochemical Research Methods ; Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology
项目资助者	Instrument Development Project of the Chinese Academy of Sciences [61334008] ; National Natural Science Foundation of China [11402274] ; Scientific and Technological Innovation "Interdiscipline and Cooperation Team" Project of the Chinese Academy of Sciences
课题组名称	LNM微纳米流体力学
论文分区	一类
引用统计	被引频次：30[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/54965
专题	非线性力学国家重点实验室
通讯作者	Yu, YD (reprint author), Chinese Acad Sci, Inst Semicond, State Key Lab Integrated Optoelect, POB 912, Beijing 100083, Peoples R China.
推荐引用方式 GB/T 7714	Sun YN,Chen XD,Zhou XG,et al. Droplet-in-oil array for picoliter-scale analysis based on sequential inkjet printing[J]. LAB ON A CHIP,2015,15,11,:2429-2436.
APA	Sun YN,陈晓东,Zhou XG,Zhu JB,Yu YD,&Yu, YD .(2015).Droplet-in-oil array for picoliter-scale analysis based on sequential inkjet printing.LAB ON A CHIP,15(11),2429-2436.
MLA	Sun YN,et al."Droplet-in-oil array for picoliter-scale analysis based on sequential inkjet printing".LAB ON A CHIP 15.11(2015):2429-2436.

条目包含的文件		下载所有文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Droplet-in-oil array（3205KB）			开放获取	--	浏览下载