| Detection of Cytomegalovirus Antibodies Using a Biosensor Based on Imaging Ellipsometry | |
Sun HL; Qi C(齐财); Niu Y(牛宇) ; Kang TF(康腾飞); Wei YX; Jin G(靳刚); Dong XZ; Wang CH; Zhu W; Zhu, W (reprint author), Shandong Acad Med Sci, Inst Radiat Med, Jinan 18877, Peoples R China.
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| Source Publication | PLOS ONE
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| 2015-08-21 | |
| Volume | 10Issue:8Pages:e0136253 |
| ISSN | 1932-6203 |
| Abstract | Background Cytomegalovirus (CMV) is the most common infectious cause of mental disability in new-borns in developed countries. There is an urgent need to establish an early detection and high-throughput screening method for CMV infection using portable detection devices. Methods An antibody analysis method is reported for the detection and identification of CMV antibodies in serum using a biosensor based on high spatial resolution imaging ellipsometry (BIE). CMV antigen (CMV-3A) was immobilized on silicon wafers and used to capture CMV antibodies in serum. An antibody against human immunoglobulin G (anti-IgG) was used to confirm the IgG antibody against CMV captured by the CMV-3A. Results Our results show that this assay is rapid and specific for the identification of IgG antibody against CMV. Further, patient serum was quantitatively assessed using the standard curve method, and the quantitative results were in agreement with the enzyme-linked immunosorbent assay. The CMV antibody detection sensitivity of BIE reached 0.01 IU/mL. Conclusions This novel biosensor may be a valuable diagnostic tool for analysis of IgG antibody against CMV during CMV infection screening. |
| DOI | 10.1371/journal.pone.0136253 |
| URL | 查看原文 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000359926900090 |
| WOS Research Area | Science & Technology - Other Topics |
| WOS Subject | Multidisciplinary Sciences |
| Funding Organization | Yantai Science and Technology Development Fund [2012128] ; Youth Backbone Teacher Fund of Binzhou Medical University ; National Natural Science Fund [81202516] ; Shandong Province Natural Science Fund [ZR2013HL039] ; Science and technology development plan of Shandong Academy of Medical Sciences [2014-08] ; Special Fund for Agro-scientific Research in the Public Interest [201303045] ; National Key Scientific Instrument and Equipment Development Project of China [2012YQ090197] ; National Basic Research Program (Project 973) of China [2009CB320300] ; National Basic Research Program of China [2009CB320302] ; National High Technology Research Development Program of China [2008AA02Z419] ; International Science & Technology Cooperation Program of China [2012DFG31880] ; Projects of Sino-Portugal ST Cooperation ; National Natural Science Foundation of China [21305147] ; Instrument Developing Project of the Chinese Academy of Sciences [KJCX2-YW-M04, KJCX2-YW-M03] |
| Department | NML纳米生物光学 |
| Classification | 二类/Q1 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/55341 |
| Collection | 微重力重点实验室 |
| Corresponding Author | Zhu, W (reprint author), Shandong Acad Med Sci, Inst Radiat Med, Jinan 18877, Peoples R China. |
| Recommended Citation GB/T 7714 | Sun HL,Qi C,Niu Y,et al. Detection of Cytomegalovirus Antibodies Using a Biosensor Based on Imaging Ellipsometry[J]. PLOS ONE,2015,10,8,:e0136253. |
| APA | Sun HL.,Qi C.,Niu Y.,Kang TF.,Wei YX.,...&Zhu, W .(2015).Detection of Cytomegalovirus Antibodies Using a Biosensor Based on Imaging Ellipsometry.PLOS ONE,10(8),e0136253. |
| MLA | Sun HL,et al."Detection of Cytomegalovirus Antibodies Using a Biosensor Based on Imaging Ellipsometry".PLOS ONE 10.8(2015):e0136253. |
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| IMCAS-J2015-119.pdf（1747KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
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