| Visualization of the interaction between tris and lysozyme with a biosensor based on total internal reflection imaging ellipsometry | |
Kang TF(康腾飞) ; Niu Y(牛宇); Jin G(靳刚); [email protected]
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| Source Publication | THIN SOLID FILMS |
| 2014 | |
| Conference Name | 6th International Conference on Spectroscopic Ellipsometry |
| Conference Date | MAY 26-31 2013 |
| Conference Place | Kyoto JAPAN |
| Abstract | Weak affinity interactions among biomolecules have attracted more and more attentions for they play a significant role in organisms. However it is difficult to characterize these interactions due to the limitation of the detection approaches. The biosensor based on total internal reflection imaging ellipsometry is a real-time analysis method for biomolecular interaction detection with the advantages of high throughput and sensitivity. The interaction between tris and lysozyme a typical example of weak affinity interactions has been detected with TIRIE as a trial. Tris is immobilized on the gold thin film substrate to form the biosensing surface and then LZM as well as its negative controls is delivered to the tris-immobilized surface. The interaction process between tris and LZM is recorded by TIRIE biosensor to forma real-time curve and its dissociation constant is deduced as 7.6 x 10 M which is in agreement with the results in the reported work by other investigators. The results indicate that TIRIE biosensor is competent for weak affinity interaction analysis. 2014 Elsevier B.V. All rights reserved. |
| Keyword | Biosensor Total Internal Reflection Imaging Ellipsometry Weak Affinity Interaction Tris Lysozyme |
| URL | 查看原文 |
| Indexed By | SCI ; CPCI |
| Language | 英语 |
| Document Type | 会议论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/55365 |
| Collection | 微重力重点实验室 |
| Corresponding Author | [email protected] |
| Recommended Citation GB/T 7714 | Kang TF,Niu Y,Jin G,et al. Visualization of the interaction between tris and lysozyme with a biosensor based on total internal reflection imaging ellipsometry[C]THIN SOLID FILMS,2014. |
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| File Name/Size | DocType | Version | Access | License | ||
| Cfp2014-012.pdf（690KB） | 会议论文 | 开放获取 | CC BY-NC-SA | View Download | ||
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