Knowledge Management System of Institue of Mechanics, CAS
| A Proteomic Study on Cell Cycle Progression of Endothelium Exposed To Tumor Conditioned Medium and the Possible Role of Cyclin D-1/E | |
Li AL; Li HW; Jin G(靳刚) ; Xiu RJ; Xiu, RJ (reprint author), Chinese Acad Med Sci, Inst Microcirculat, Beijing 100005, Peoples R China.
| |
| Source Publication | CLINICAL HEMORHEOLOGY AND MICROCIRCULATION
 |
| 2003 | |
| Volume | 29Issue:3-4Pages:383-390 |
| ISSN | 1386-0291 |
| Abstract | This study was designed to comprehensively analyze the differential expression of proteins from human umbilical vein endothelial cells (HUVECs) exposed to tumor conditioned medium (TCM) and to identify the key regulator in the cell cycle progression. The HUVECs were exposed to TCM from breast carcinoma cell line MDA-MB-231, then their cell cycle distribution was measured by flow cytometer (FCM). The role of protein in cell cycle progression was detected via two-dimensional polyacrylamide gel electrophoresis (2-DE) and western blotting. Following the stimulation of TCM, HUVECs showed a more cells in the S phase than did the negative control group (ECGF-free medium with 20% FBS), but the HUVECs' level was similar to the positive control group (medium with 25 mug/ml ECGF and 20% FBS). Increased expression of cyclin D-1/E and some changes in other related proteins occurred after incubation with TCM. From our results, we can conclude that breast carcinoma cell line MDA-MB-231 may secrete soluble pro-angiogenic factors that induce the HUVEC angiogenic switch, during which the expression of cell cycle regulator cyclin D-1/E increases and related proteins play an important role in this process. |
| Keyword | Tumor Conditioned Medium Endothelium Cell Cycle 2-de Western Blot |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000188240800032 |
| WOS Keyword | GROWTH-FACTOR ; CANCER-CELLS ; AGENT TNP-470 ; IN-VITRO ; ANGIOGENESIS ; ANTISENSE ; D1 ; INHIBITION ; P21(WAF1/CIP1) ; TUMORIGENICITY |
| WOS Research Area | Hematology ; Cardiovascular System & Cardiology |
| WOS Subject | Hematology ; Peripheral Vascular Disease |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/58477 |
| Collection | 力学所知识产出(1956-2008) |
| Corresponding Author | Xiu, RJ (reprint author), Chinese Acad Med Sci, Inst Microcirculat, Beijing 100005, Peoples R China. |
| Recommended Citation GB/T 7714 | Li AL,Li HW,Jin G,et al. A Proteomic Study on Cell Cycle Progression of Endothelium Exposed To Tumor Conditioned Medium and the Possible Role of Cyclin D-1/E[J]. CLINICAL HEMORHEOLOGY AND MICROCIRCULATION,2003,29,3-4,:383-390. |
| APA | Li AL,Li HW,靳刚,Xiu RJ,&Xiu, RJ .(2003).A Proteomic Study on Cell Cycle Progression of Endothelium Exposed To Tumor Conditioned Medium and the Possible Role of Cyclin D-1/E.CLINICAL HEMORHEOLOGY AND MICROCIRCULATION,29(3-4),383-390. |
| MLA | Li AL,et al."A Proteomic Study on Cell Cycle Progression of Endothelium Exposed To Tumor Conditioned Medium and the Possible Role of Cyclin D-1/E".CLINICAL HEMORHEOLOGY AND MICROCIRCULATION 29.3-4(2003):383-390. |
| Files in This Item: | Download All | |||||
| File Name/Size | DocType | Version | Access | License | ||
| JourArSuppl2016-196.（77KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Download | |
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment