| Detection of Duck Hepatitis Virus Serotype1 By Biosensor Based on Imaging Ellipsometry | |
Huang CH; Li JX ; Tang Y; Chen YY(陈艳艳); Jin G(靳刚); Jin, G (reprint author), CAS, Suzhou Inst Nanotech & Nanobion, Suzhou 215125, Peoples R China.
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| Source Publication | CURRENT APPLIED PHYSICS
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| 2011 | |
| Volume | 11Issue:3Pages:353-357 |
| ISSN | 1567-1739 |
| Abstract | A novel method for the detection of duck hepatitis virus serotype1 (DHV1) using a biosensor based on imaging ellipsometry (IE) is developed. Polyclonal antibodies against DHV1 were immobilized on silicon wafers through protein A and used to capture virus particles in sample. Signals on silicon surface were acquired directly in grayscale format on an imaging ellipsometry image. This study demonstrated that the assay is rapid and accurate for detection of duck hepatitis virus. Compared with traditional methods, this technique not only has high sensitivity as low as 8 x 10(-9.5) LD(50)/ml, but can also simultaneously perform multiple serotype identification of DHV1, DHV1-g and DHV1-s. These results suggest that this biosensor might be a practicable diagnostic tool for duck hepatitis virus detection. (C) 2010 Elsevier B.V. All rights reserved. |
| Keyword | Biosensor Imaging Ellipsometry Duck Hepatitis Virus Detection |
| DOI | 10.1016/j.cap.2010.08.004 |
| Indexed By | SCI ; EI |
| Language | 英语 |
| WOS ID | WOS:000288183300017 |
| WOS Keyword | VISUALIZATION |
| WOS Research Area | Materials Science ; Physics |
| WOS Subject | Materials Science, Multidisciplinary ; Physics, Applied |
| Funding Organization | Ministry of Science and Technology of China [2008AA02Z419] ; Natural Science Foundation of China [10804083, 20845003] ; Chinese Academy of Sciences [KJCX2.YW.M04] ; Key Laboratory of Microgravity, Institute of Mechanics, CAS ; southwest University, Chongqing, China |
| Department | NML纳米生物光学 |
| Classification | 二类 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/58588 |
| Collection | 微重力重点实验室 |
| Corresponding Author | Jin, G (reprint author), CAS, Suzhou Inst Nanotech & Nanobion, Suzhou 215125, Peoples R China. |
| Recommended Citation GB/T 7714 | Huang CH,Li JX,Tang Y,et al. Detection of Duck Hepatitis Virus Serotype1 By Biosensor Based on Imaging Ellipsometry[J]. CURRENT APPLIED PHYSICS,2011,11,3,:353-357. |
| APA | Huang CH,Li JX,Tang Y,陈艳艳,靳刚,&Jin, G .(2011).Detection of Duck Hepatitis Virus Serotype1 By Biosensor Based on Imaging Ellipsometry.CURRENT APPLIED PHYSICS,11(3),353-357. |
| MLA | Huang CH,et al."Detection of Duck Hepatitis Virus Serotype1 By Biosensor Based on Imaging Ellipsometry".CURRENT APPLIED PHYSICS 11.3(2011):353-357. |
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| JourArSuppl2016-020.（572KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Download | |
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