| Label-free multiplex immunoassay of AFP, CEA and CA19-9 by integrated microfluidic biosensor based on imaging ellipsometry | |
Huang CH; Yang QL; Song FY; Chen N ; Yao B; Liao XL; Zhang ST; Chen YY; Jin G(靳刚); Huang, CH (reprint author), Chongqing Univ Sci & Technol, Inst Biomat & Living Cell Imaging Technol, Chongqing, Peoples R China.; Chen, YY (reprint author), Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou, Peoples R China.; Jin, G (reprint author), Chinese Acad Sci, Inst Mech, Beijing, Peoples R China.
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| Source Publication | INTEGRATED FERROELECTRICS
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| 2016 | |
| Volume | 171Issue:1Pages:59-69 |
| ISSN | 1058-4587 |
| Abstract | This study describes the development of alpha-fetoprotein (AFP), carcinoembryonic antigen (CEA) and carbohydrate 19-9(CA19-9) multiplex immunoassay by integrated microfluidic device based on imaging ellipsometry (IMBIE). Different strategies for AFP, CEA and CA 19-9 simultaneous immunoassay on the same aldehyde-substrate were developed. Unifying the reaction steps of CA19-9 for antigen direct detection, CEA for secondary antibody (rabbit-antiCEA) addition, AFP for secondary (rabbit antiAFP) and an enhancer (goat IgG) application, and comprehensively considering the dynamic ranges and detection sensitivities, we combined these three markers into multiplex analysis in serial format.The sensitivities and dynamic ranges for CA19-9, CEA and AFP were 10.0U/mL, 1.0 ng/mL, 1.0 ng/mL and 2.0-100.0 U/mL, 0.1-64.0 ng/mL, 1.0-128.0 ng/mL, respectively. The intra-assay and inter-assay (coefficient variations) CVs of CA19-9, CEA and AFP were between 1.6-8.9% and 2.1-7.8%. The specificities tested for both cross-reaction and heterogeneous proteins of CA19-9, CEA and AFP were not more than 6.7%. Quantitative detection of 49 sera from gastric patients demonstrated that the accuracy for gastric cancer diagnosis was 83.4%. The total detection time is only 60min for an automated process. The proposed assay has the potential for practical application. |
| Keyword | Imaging Ellipsometry Immunoassay Afp Cea Ca19-9 |
| DOI | 10.1080/10584587.2016.1171664 |
| URL | 查看原文 |
| Indexed By | SCI ; EI |
| Language | 英语 |
| WOS ID | WOS:000378406600009 |
| WOS Keyword | Imaging ellipsometry ; immunoassay ; AFP ; CEA ; CA19-9 |
| WOS Research Area | Engineering ; Physics |
| WOS Subject | Engineering, Electrical & Electronic ; Physics, Applied ; Physics, Condensed Matter |
| Funding Organization | This work is supported by the cooperative project of academician workstation of Chongqing University of Science & Technology (CKYS2014Y5), Research Foundation of Chongqing University of Science & Technology (CK2014B4) and Chongqing pogram of Application Foundation and Advanced Technology(cstc2014jcyjA50032 cstc2014jcyjA10029). |
| Department | NML纳米生物光学 |
| Classification | Q4 |
| Ranking | False |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/59532 |
| Collection | 微重力重点实验室 |
| Corresponding Author | Huang, CH (reprint author), Chongqing Univ Sci & Technol, Inst Biomat & Living Cell Imaging Technol, Chongqing, Peoples R China.; Chen, YY (reprint author), Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou, Peoples R China.; Jin, G (reprint author), Chinese Acad Sci, Inst Mech, Beijing, Peoples R China. |
| Recommended Citation GB/T 7714 | Huang CH,Yang QL,Song FY,et al. Label-free multiplex immunoassay of AFP, CEA and CA19-9 by integrated microfluidic biosensor based on imaging ellipsometry[J]. INTEGRATED FERROELECTRICS,2016,171,1,:59-69. |
| APA | Huang CH.,Yang QL.,Song FY.,Chen N.,Yao B.,...&Jin, G .(2016).Label-free multiplex immunoassay of AFP, CEA and CA19-9 by integrated microfluidic biosensor based on imaging ellipsometry.INTEGRATED FERROELECTRICS,171(1),59-69. |
| MLA | Huang CH,et al."Label-free multiplex immunoassay of AFP, CEA and CA19-9 by integrated microfluidic biosensor based on imaging ellipsometry".INTEGRATED FERROELECTRICS 171.1(2016):59-69. |
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| a2016-061.pdf（703KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Download | |
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