| The Effect of Attractive Interactions and Macromolecular Crowding on Crystallins Association | |
Wei JC(韦佳辰); Dobnikar J; Curk T; Song F(宋凡) ; Song, F (reprint author), Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech LNM, Beijing 100190, Peoples R China.
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| Source Publication | PLOS ONE
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| 2016 | |
| Volume | 11Issue:3Pages:e0151159 |
| ISSN | 1932-6203 |
| Abstract | In living systems proteins are typically found in crowded environments where their effective interactions strongly depend on the surrounding medium. Yet, their association and dissociation needs to be robustly controlled in order to enable biological function. Uncontrolled protein aggregation often causes disease. For instance, cataract is caused by the clustering of lens proteins, i.e., crystallins, resulting in enhanced light scattering and impaired vision or blindness. To investigate the molecular origins of cataract formation and to design efficient treatments, a better understanding of crystallin association in macromolecular crowded environment is needed. Here we present a theoretical study of simple coarse grained colloidal models to characterize the general features of how the association equilibrium of proteins depends on the magnitude of intermolecular attraction. By comparing the analytic results to the available experimental data on the osmotic pressure in crystallin solutions, we identify the effective parameters regimes applicable to crystallins. Moreover, the combination of two models allows us to predict that the number of binding sites on crystallin is small, i.e. one to three per protein, which is different from previous estimates. We further observe that the crowding factor is sensitive to the size asymmetry between the reactants and crowding agents, the shape of the protein clusters, and to small variations of intermolecular attraction. Our work may provide general guidelines on how to steer the protein interactions in order to control their association. |
| DOI | 10.1371/journal.pone.0151159 |
| URL | 查看原文 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000371991300090 |
| WOS Research Area | Science & Technology - Other Topics |
| WOS Subject | Multidisciplinary Sciences |
| Funding Organization | This work was supported by the National Natural Science Foundation of China (Grant No. 11232013 and No. 11472285) and the Fundamental Research Funds for the Central Universities of P. R. China. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. ; This work was supported by the National Natural Science Foundations of China ( Grants No. 11232013 and No. 11472285) and the Fundamental Research Funds for the Central Universities of P. R. China under the project buctrc201422. |
| Department | LNM生物材料微结构和力学性能 |
| Classification | 二类 |
| Ranking | True |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/59570 |
| Collection | 非线性力学国家重点实验室 |
| Corresponding Author | Song, F (reprint author), Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech LNM, Beijing 100190, Peoples R China. |
| Recommended Citation GB/T 7714 | Wei JC,Dobnikar J,Curk T,et al. The Effect of Attractive Interactions and Macromolecular Crowding on Crystallins Association[J]. PLOS ONE,2016,11,3,:e0151159. |
| APA | Wei JC,Dobnikar J,Curk T,Song F,&Song, F .(2016).The Effect of Attractive Interactions and Macromolecular Crowding on Crystallins Association.PLOS ONE,11(3),e0151159. |
| MLA | Wei JC,et al."The Effect of Attractive Interactions and Macromolecular Crowding on Crystallins Association".PLOS ONE 11.3(2016):e0151159. |
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| JouAr-2017-084.PDF（2871KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Download | |
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