| Changes in tension regulates proliferation and migration of fibroblasts by remodeling expression of ECM proteins | |
Jiang MM; Qiu JH; Zhang LL; Lu DY; Long M(龙勉) ; Che, L; Luo XD; Luo, XD (reprint author), Third Mil Med Univ, Burn Res Inst, Southwest Hosp, 30 Gaotanyan St, Chongqing 400038, Peoples R China.; Chen, L (reprint author), Third Mil Med Univ, Southwest Hosp, Breast Dis Ctr, 30 Gaotanyan St, Chongqing 400038, Peoples R China.
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| Source Publication | EXPERIMENTAL AND THERAPEUTIC MEDICINE
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| 2016 | |
| Volume | 12Issue:3Pages:1542-1550 |
| ISSN | 1792-0981 |
| Abstract | Wound healing is a complicated but highly organized process in which cell migration and proliferation are actively involved. However, the process by which mechanical stretch regulates the proliferation and migration of human skin fibroblasts (HFs) and keratinocytes is poorly understood. Using a house built mechanical stretch device, we examined the HFs extracellular matrix (ECM) components changes under non-stretch, static stretch or cyclic stretch conditions. We further investigated the changes in ECM component protein expression levels in keratinocytes and analyzed the effects of individual ECM component on keratinocyte proliferation and migration. Particularly, the roles of calcium/calmodulin-dependent serine protein kinase (CASK) in the HF proliferation under cyclic stretch were investigated. Cyclic stretch suppressed HF proliferation compared with HFs without stretch or with static stretch. Cyclic stretch also led to a significant reduction in the levels of collagen I and a marked increase of fibronectin in HFs ECM. By contrast, collagen I levels increased and fibronectin levels decreased in response to non-stretch and static stretch conditions. After cyclic stretch, the proliferation of keratinocytes was inhibited by the cyclic stretch-induced ECM in HFs. The inoculation of keratinocytes with single ECM component suggested that collagen I was more capable of inducing cell proliferation than fibronectin, while it had less impact on cell migration compared with fibronectin. Furthermore, cyclic stretch induced by proliferation inhibition was associated with altered integrin 1-CASK signal pathway. The present results demonstrated the existence of HF-ECM-keratinocyte cross-talk' in cutaneous tissues. Thus, the integrin 1-CASK signal pathway in HFs may be involved in the outside-in signal transduction of extracellular stretch and the altered ECM component expression. |
| Keyword | Human Skin Fibroblast Keratinocyte Mechanical Stretch Extracellular Matrix Integrin Calcium Calmodulin-dependent Serine Protein Kinase |
| DOI | 10.3892/etm.2016.3497 |
| URL | 查看原文 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000382446000047 |
| WOS Keyword | human skin fibroblast ; keratinocyte ; mechanical stretch ; extracellular matrix ; integrin ; calcium ; calmodulin-dependent serine protein kinase |
| WOS Research Area | Research & Experimental Medicine |
| WOS Subject | Medicine, Research & Experimental |
| Funding Organization | This study was supported by grants from the National High Technology Research and Development Program of China (grant no. 2011CB710904) and the National Natural Science Foundation of China (grant no. 81372813). |
| Department | NML分子-细胞生物力学与空间生命科学 |
| Classification | Q4 |
| Ranking | False |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/59743 |
| Collection | 微重力重点实验室 |
| Corresponding Author | Luo, XD (reprint author), Third Mil Med Univ, Burn Res Inst, Southwest Hosp, 30 Gaotanyan St, Chongqing 400038, Peoples R China.; Chen, L (reprint author), Third Mil Med Univ, Southwest Hosp, Breast Dis Ctr, 30 Gaotanyan St, Chongqing 400038, Peoples R China. |
| Recommended Citation GB/T 7714 | Jiang MM,Qiu JH,Zhang LL,et al. Changes in tension regulates proliferation and migration of fibroblasts by remodeling expression of ECM proteins[J]. EXPERIMENTAL AND THERAPEUTIC MEDICINE,2016,12,3,:1542-1550. |
| APA | Jiang MM.,Qiu JH.,Zhang LL.,Lu DY.,龙勉.,...&Chen, L .(2016).Changes in tension regulates proliferation and migration of fibroblasts by remodeling expression of ECM proteins.EXPERIMENTAL AND THERAPEUTIC MEDICINE,12(3),1542-1550. |
| MLA | Jiang MM,et al."Changes in tension regulates proliferation and migration of fibroblasts by remodeling expression of ECM proteins".EXPERIMENTAL AND THERAPEUTIC MEDICINE 12.3(2016):1542-1550. |
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| a2016-054.pdf（1118KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Download | |
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