| Mechanical remodeling of normally sized mammalian cells under a gravity vector | |
Zhang C ; Zhou LW(周吕文); Zhang F(张帆); Lv DY(吕东媛); Li N(李宁); Zheng L; Xu YH(徐艳红); Li Z; Sun SJ(孙树津); Long M(龙勉); Long, M (reprint author), Chinese Acad Sci, Inst Mech, 15 North 4th Ring Rd, Beijing 100190, Peoples R China.
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| Source Publication | FASEB JOURNAL
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| 2017-02-01 | |
| Volume | 31Issue:2Pages:802-813 |
| ISSN | 0892-6638 |
| Abstract | Translocation of the dense nucleus along a gravity vector initiates mechanical remodeling of a cell but the underlying mechanisms of cytoskeletal network and focal adhesion complex (FAC) reorganization in a mammalian cell remain unclear. We quantified the remodeling of an MC3T3-E1 cell placed in upward- downward- or edge-on-orientated substrate. Nucleus longitudinal translocation presents a high value in downward orientation at 24 h or in edge-on orientation at 72 h which is consistent with orientation-dependent distribution of perinuclear actin stress fibers and vimentin cords. Redistribution of total FAC area and fractionized super mature adhesion number coordinates this dependence at short duration. This orientation-dependent remodeling is associated with nucleus flattering and lamin A/C phosphorylation. Actin depolymerization or Rho-associated protein kinase signaling inhibition abolishes the orientation dependence of nucleus translocation whereas tubulin polymerization inhibition or vimentin disruption reserves the dependence. A biomechanical model is therefore proposed for integrating the mechanosensing of nucleus translocation with cytoskeletal remodeling and FAC reorganization induced by a gravity vector. |
| Keyword | Gravity-directed Mechanosensing Nucleus Translocation Cytoskeletal Remodeling Fac Reorganization |
| DOI | 10.1096/fj.201600897RR |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000394235400032 |
| WOS Keyword | FOCAL ADHESION DYNAMICS ; ROS 17/2.8 CELLS ; NANOSCALE ARCHITECTURE ; ACTIN ; FORCE ; MECHANOTRANSDUCTION ; CYTOSKELETON ; STRESS ; TALIN ; MICROTUBULES |
| WOS Research Area | Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics ; Cell Biology |
| WOS Subject | Biochemistry & Molecular Biology ; Biology ; Cell Biology |
| Funding Organization | National Key Basic Research Foundation of China(2011CB710904) ; Strategic Priority Research Program(XDA04020219 ; National Natural Science Foundation of China(31110103918) ; National High Technology Research and Development Program of China(2011AA020109) ; XDA01030102) |
| Department | NML分子-细胞生物力学与空间生命科学 |
| Classification | 一类 |
| Ranking | 1 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/60581 |
| Collection | 微重力重点实验室 |
| Corresponding Author | Long, M (reprint author), Chinese Acad Sci, Inst Mech, 15 North 4th Ring Rd, Beijing 100190, Peoples R China. |
| Recommended Citation GB/T 7714 | Zhang C,Zhou LW,Zhang F,et al. Mechanical remodeling of normally sized mammalian cells under a gravity vector[J]. FASEB JOURNAL,2017,31,2,:802-813. |
| APA | Zhang C.,周吕文.,张帆.,吕东媛.,李宁.,...&Long, M .(2017).Mechanical remodeling of normally sized mammalian cells under a gravity vector.FASEB JOURNAL,31(2),802-813. |
| MLA | Zhang C,et al."Mechanical remodeling of normally sized mammalian cells under a gravity vector".FASEB JOURNAL 31.2(2017):802-813. |
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| JouAr-2017-075.pdf（3686KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Download | |
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