| Mimicking liver sinusoidal structures and functions using a 3D-configured microfluidic chip | |
Du Y(杜宇) ; Li N(李宁); Yang H(杨浩); Luo CH(罗春花); Gong YX; Tong CF(佟春芳); Gao YX(高宇新); Lv SQ(吕守芹); Long M(龙勉); Long, M (reprint author), Chinese Acad Sci, Key Lab Micrograv, Natl Micrograv Lab, Ctr Biomech & Bioengn, Beijing 100190, Peoples R China.; Long, M (reprint author), Chinese Acad Sci, Beijing Key Lab Engn Construct & Mech, Inst Mech, Beijing 100190, Peoples R China.; Long, M (reprint author), Univ Chinese Acad Sci, Sch Engn Sci, Beijing 100049, Peoples R China.
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| Source Publication | LAB ON A CHIP
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| 2017 | |
| Volume | 17Issue:5Pages:782-794 |
| ISSN | 1473-0197 |
| Abstract | Physiologically four major types of hepatic cells - the liver sinusoidal endothelial cells Kupffer cells hepatic stellate cells and hepatocytes - reside inside liver sinusoids and interact with flowing peripheral cells under blood flow. It is hard to mimic an in vivo liver sinusoid due to its complex multiple cell-cell interactions spatiotemporal construction and mechanical microenvironment. Here we developed an in vitro liver sinusoid chip by integrating the four types of primary murine hepatic cells into two adjacent fluid channels separated by a porous permeable membrane replicating liver's key structures and configurations. Each type of cells was identified with its respective markers and the assembled chip presented the liver-specific unique morphology of fenestration. The flow field in the liver chip was quantitatively analyzed by computational fluid dynamics simulations and particle tracking visualization tests. Intriguingly co-culture and shear flow enhance albumin secretion independently or cooperatively while shear flow alone enhances HGF production and CYP450 metabolism. Under lipopolysaccharide (LPS) stimulations the hepatic cell co-culture facilitated neutrophil recruitment in the liver chip. Thus this 3D-configured in vitro liver chip integrates the two key factors of shear flow and the four types of primary hepatic cells to replicate key structures hepatic functions and primary immune responses and provides a new in vitro model to investigate the short-duration hepatic cellular interactions under a microenvironment mimicking the physiology of a liver. |
| DOI | 10.1039/c6lc01374k |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000395893500003 |
| WOS Keyword | ON-A-CHIP ; ENDOTHELIAL-CELLS ; IN-VITRO ; DIFFERENTIAL REGULATION ; METABOLIC-ACTIVITY ; STEM-CELLS ; RAT-LIVER ; FLOW ; HEPATOCYTES ; MORPHOLOGY |
| WOS Research Area | Biochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics |
| WOS Subject | Biochemical Research Methods ; Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology |
| Funding Organization | National Natural Science Foundation of China(31230027 ; CAS Strategic Priority Research Program(XDA01030604 ; 91642203 ; XDB22040101) ; 31661143044 ; 31110103918) |
| Department | NML分子-细胞生物力学与空间生命科学 |
| Classification | 一类 |
| Ranking | 1 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/60599 |
| Collection | 微重力重点实验室 |
| Corresponding Author | Long, M (reprint author), Chinese Acad Sci, Key Lab Micrograv, Natl Micrograv Lab, Ctr Biomech & Bioengn, Beijing 100190, Peoples R China.; Long, M (reprint author), Chinese Acad Sci, Beijing Key Lab Engn Construct & Mech, Inst Mech, Beijing 100190, Peoples R China.; Long, M (reprint author), Univ Chinese Acad Sci, Sch Engn Sci, Beijing 100049, Peoples R China. |
| Recommended Citation GB/T 7714 | Du Y,Li N,Yang H,et al. Mimicking liver sinusoidal structures and functions using a 3D-configured microfluidic chip[J]. LAB ON A CHIP,2017,17,5,:782-794. |
| APA | 杜宇.,李宁.,杨浩.,罗春花.,Gong YX.,...&Long, M .(2017).Mimicking liver sinusoidal structures and functions using a 3D-configured microfluidic chip.LAB ON A CHIP,17(5),782-794. |
| MLA | 杜宇,et al."Mimicking liver sinusoidal structures and functions using a 3D-configured microfluidic chip".LAB ON A CHIP 17.5(2017):782-794. |
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| File Name/Size | DocType | Version | Access | License | ||
| JouAr-2017-024.pdf（6728KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View Download | |
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