| Site-directed immobilization antibody for alpha-fetoprotein detection by optical biosensor |
| Huang, Chenghong; Yang, QingLin; Song, Fayi; Chen, Nan; Liao, Xiaoling; Yao, Bo; Zhang, ShiTong; Chen, Yanyan; Jin G(靳刚) ; Huang, CH (reprint author), Chongqing Univ Sci & Technol, Chongqing, Peoples R China.; Chen, YY (reprint author), Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou, Peoples R China.; Jin, G (reprint author), Chinese Acad Sci, Inst Mech, Beijing, Peoples R China.
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| Source Publication | INTEGRATED FERROELECTRICS
(IF:0.486[JCR-2018],0.563[5-Year]) |
| 2016
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| Volume | 171Issue:1Pages:70-78 |
| ISSN | 1058-4587
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| Abstract | Carbohydrate side chain in antibody Fc region can be oxidized into aldehyde group by sodium m-periodate, which can simultaneously couple with the amino-group on silicon surface obtained by chemistry modification. This combination will introduce C=N linkage and site-directed immobilization of antibody, which was confirmed by AFM observation. The acquired antibody layer can then be used for immunoassay for alpha-fetoprotein (AFP). The combined antigen is directly detected by imaging ellipsometry in grayscale format. The proposed method was further compared with commercial kit by determination 20 clinic samples with significant correlation (R = 0.93). |
| Keyword | Alpha-fetoprotein
Imaging Ellipsometry
Immunoassay
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| DOI | 10.1080/10584587.2016.1172004
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| Indexed By | SCI
; EI
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| Language | 英语
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| WOS ID | WOS:000378406600010
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| WOS Keyword | ATOMIC-FORCE MICROSCOPY
; IMAGING ELLIPSOMETRY
; VISUALIZATION
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| WOS Research Area | Engineering, Electrical & Electronic
; Physics, Applied
; Physics, Condensed Matter
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| Funding Organization | This work is supported by the cooperative project of academician workstation of Chongqing University of Science & Technology (CKYS2014Y5), Research Foundation of Chongqing University of Science & Technology (CK2014B4) and Chongqing pogram of Application Foundation and Advanced Technology(cstc2014jcyjA50032, cstc2014jcyjA10029).
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| Department | NML纳米生物光学
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| Classification | Q4
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| Citation statistics |
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| Document Type | 期刊论文
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| Identifier | http://dspace.imech.ac.cn/handle/311007/71955
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| Collection | 微重力重点实验室
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| Corresponding Author | Huang, CH (reprint author), Chongqing Univ Sci & Technol, Chongqing, Peoples R China.; Chen, YY (reprint author), Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou, Peoples R China.; Jin, G (reprint author), Chinese Acad Sci, Inst Mech, Beijing, Peoples R China. |
Recommended Citation
GB/T 7714 |
Huang, Chenghong,Yang, QingLin,Song, Fayi,et al. Site-directed immobilization antibody for alpha-fetoprotein detection by optical biosensor[J]. INTEGRATED FERROELECTRICS,2016,171,1,:70-78.
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| APA |
Huang, Chenghong.,Yang, QingLin.,Song, Fayi.,Chen, Nan.,Liao, Xiaoling.,...&Jin, G .(2016).Site-directed immobilization antibody for alpha-fetoprotein detection by optical biosensor.INTEGRATED FERROELECTRICS,171(1),70-78.
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| MLA |
Huang, Chenghong,et al."Site-directed immobilization antibody for alpha-fetoprotein detection by optical biosensor".INTEGRATED FERROELECTRICS 171.1(2016):70-78.
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