| Binding of intercellular adhesion molecule 1 to beta(2)-integrin regulates distinct cell adhesion processes on hepatic and cerebral endothelium | |
Tong CF(佟春芳) ; Zhang Y(章燕); Lv SQ(吕守芹); Li N(李宁); Gong YX(龚一心); Yang H; Feng SL; Du Y; Huang DD; Long M(龙勉)
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| Source Publication | AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
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| 2018-09-01 | |
| Volume | 315Issue:3Pages:C409-C421 |
| ISSN | 0363-6143 |
| Abstract | Flowing polymorphonuclear neutrophils (PMNs) are forced to recruit toward inflamed tissue and adhere to vascular endothelial cells, which is primarily mediated by the binding of beta(2)-integrins to ICAM-1. This process is distinct among different organs such as liver and brain; however, the underlying kinetic and mechanical mechanisms regulating tissue-specific recruitment of PMNs remain unclear. Here, binding kinetics measurement showed that ICAM-1 on murine hepatic sinusoidal endothelial cells (LSECs) bound to lymphocyte function-associated antigen-1 (LFA-1) with higher on-and off-rates but lower effective affinity compared with macrophage-1 antigen (Mac-1), whereas ICAM-1 on cerebral endothelial cells (BMECs or bEnd. 3 cells) bound to LFA-1 with higher on-rates, similar off-rates, and higher effective affinity compared with Mac-1. Physiologically, free crawling tests of PMN onto LSEC, BMEC, or bEnd. 3 monolayers were consistent with those kinetics differences between two beta(2)-integrins interacting with hepatic sinusoid or cerebral endothelium. Numerical calculations and Monte Carlo simulations validated tissue-specific contributions of beta(2)-integrin-ICAM-1 kinetics to PMN crawling on hepatic sinusoid or cerebral endothelium. Thus, this work first quantified the biophysical regulation of PMN adhesion in hepatic sinusoids compared with cerebral endothelium. |
| Keyword | brain ICAM-1 integrin kinetics liver |
| DOI | 10.1152/ajpcell.00083.2017 |
| URL | 查看原文 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000444021100014 |
| WOS Keyword | BLOOD-BRAIN-BARRIER ; 2-DIMENSIONAL KINETICS ; NEUTROPHIL RECRUITMENT ; LEUKOCYTE RECRUITMENT ; LIVER METASTASIS ; INFLAMMATION ; SELECTIN ; MOTILITY ; MICROPIPETTE ; MECHANICS |
| WOS Research Area | Cell Biology ; Physiology |
| WOS Subject | Cell Biology ; Physiology |
| Funding Organization | National Natural Science Foundation of China [31230027, 91642203, 31661143044] ; Chinese Academy of Sciences Strategic Priority Research Program [XDB22040101] ; Frontier Science Key Project [QYZDJ-SSW-JSC018] ; National Key Research and Development Program of China [2016YFA0501601] |
| Classification | 二类 |
| Ranking | 1 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/77952 |
| Collection | 微重力重点实验室 |
| Affiliation | 1.[Tong, Chun-Fang 2.Zhang, Yan 3.Lu, Shou-Qin 4.Li, Ning 5.Gong, Yi-Xin 6.Yang, Hao 7.Feng, Shi-Liang 8.Du, Yu 9.Huang, Dan-Dan 10.Chinese Acad Sci, Natl Micrograv Lab, Ctr Biomech & Bioengn, Key Lab Micrograv, Beijing, Peoples R China 11.[Tong, Chun-Fang 12.Zhang, Yan 13.Lu, Shou-Qin 14.Li, Ning 15.Gong, Yi-Xin 16.Yang, Hao 17.Feng, Shi-Liang 18.Du, Yu 19.Huang, Dan-Dan 20.Chinese Acad Sci, Beijing Key Lab Engn Construct & Mechanobiol, Inst Mech, Beijing, Peoples R China 21.[Zhang, Yan 22.Lu, Shou-Qin 23.Univ Chinese Acad Sci, Sch Engn Sci, Beijing, Peoples R China |
| Recommended Citation GB/T 7714 | Tong CF,Zhang Y,Lv SQ,et al. Binding of intercellular adhesion molecule 1 to beta(2)-integrin regulates distinct cell adhesion processes on hepatic and cerebral endothelium[J]. AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY,2018,315,3,:C409-C421. |
| APA | 佟春芳.,章燕.,吕守芹.,李宁.,龚一心.,...&龙勉.(2018).Binding of intercellular adhesion molecule 1 to beta(2)-integrin regulates distinct cell adhesion processes on hepatic and cerebral endothelium.AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY,315(3),C409-C421. |
| MLA | 佟春芳,et al."Binding of intercellular adhesion molecule 1 to beta(2)-integrin regulates distinct cell adhesion processes on hepatic and cerebral endothelium".AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY 315.3(2018):C409-C421. |
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| IrJ2018341.pdf（1593KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
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