| Liver sinusoid on a chip | |
Du Y(杜宇)1,2 ; Li N(李宁)1,2,3; Long M(龙勉)1,2,3
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| Corresponding Author | Long, Mian([email protected]) |
| Source Publication | MICROFLUIDICS IN CELL BIOLOGY, PT A: MICROFLUIDICS FOR MULTICELLULAR SYSTEMS
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| 2018 | |
| Volume | 146Pages:105-134 |
| ISSN | 0091-679X |
| Abstract | Liver sinusoid is the main functional site in liver. Multiple types of hepatic cells are well organized in a precisely-controlled biochemical and biomechanical environment, maintaining a spectrum of hepatic functions. Here, using micro-engineering techniques, four types of primary hepatic cells are integrated into two layer channels connected by porous membrane, which recreates the sinusoidal cell composition and architecture. By incorporating shear flow into this permeable system, the blood flow in sinusoids and interstitial flow in space of Disse are recapitulated. Conventional hepatocyte-based liver-specific functions are enhanced by non-parenchymal cells co-culture and shear flow. Moreover, major immune responses in liver sinusoids, i.e., neutrophil recruitment under lipopolysaccharide (LPS) stimulation, are replicated, indicating that all types of hepatic cells contribute to this process. Thus, this liver chip provides a new in vitro model to investigate the short-duration cellular interactions under a microenvironment mimicking the physiological composition and architecture of liver organ. |
| DOI | 10.1016/bs.mcb.2018.06.002 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000452411700007 |
| WOS Keyword | ENDOTHELIAL-CELLS ; INFLAMED LIVER ; KUPFFER CELLS ; GENE-EXPRESSION ; CXC CHEMOKINES ; FLOW DYNAMICS ; INJURY ; NEUTROPHILS ; MECHANISMS ; LYMPHOCYTES |
| WOS Research Area | Cell Biology |
| WOS Subject | Cell Biology |
| Classification | Q4 |
| Ranking | 1 |
| Contributor | Long, Mian |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/78961 |
| Collection | 微重力重点实验室 |
| Affiliation | 1.Chinese Acad Sci, Ctr Biomech & Bioengn, Key Lab Micrograv, Natl Micrograv Lab, Beijing, Peoples R China; 2.Chinese Acad Sci, Inst Mech, Beijing Key Lab Engn Construct & Mechanobiol, Beijing, Peoples R China; 3.Univ Chinese Acad Sci, Sch Engn Sci, Beijing, Peoples R China |
| Recommended Citation GB/T 7714 | Du Y,Li N,Long M. Liver sinusoid on a chip[J]. MICROFLUIDICS IN CELL BIOLOGY, PT A: MICROFLUIDICS FOR MULTICELLULAR SYSTEMS,2018,146:105-134.Rp_Au:Long, Mian |
| APA | 杜宇,李宁,&龙勉.(2018).Liver sinusoid on a chip.MICROFLUIDICS IN CELL BIOLOGY, PT A: MICROFLUIDICS FOR MULTICELLULAR SYSTEMS,146,105-134. |
| MLA | 杜宇,et al."Liver sinusoid on a chip".MICROFLUIDICS IN CELL BIOLOGY, PT A: MICROFLUIDICS FOR MULTICELLULAR SYSTEMS 146(2018):105-134. |
| Files in This Item: | Download All | |||||
| File Name/Size | DocType | Version | Access | License | ||
| Jp2019167.pdf（3794KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
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