| Mechanical Point Loading Induces Cortex Stiffening and Actin Reorganization | |
Hu JR(胡锦荣)1,2,3 ; Chen SB(陈深宝)1,2,3; Hu WH(胡文慧)1,2,4; Lv SQ(吕守芹)1,2,3; Long M(龙勉)1,2,3
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| Corresponding Author | Lu, Shouqin([email protected]) ; Long, Mian([email protected]) |
| Source Publication | BIOPHYSICAL JOURNAL
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| 2019-10-15 | |
| Volume | 117Issue:8Pages:1405-1418 |
| ISSN | 0006-3495 |
| Abstract | Global cytoskeleton reorganization is well-recognized when cells are exposed to distinct mechanical stimuli, but the localized responses at a specified region of a cell are still unclear. In this work, we mapped the cell-surface mechanical property of single cells in situ before and after static point loading these cells using atomic force microscopy in PeakForce-Quantitative Nano Mechanics mode. Cell-surface stiffness was elevated at a maximum of 1.35-fold at the vicinity of loading site, indicating an enhanced structural protection of the cortex to the cell. Mechanical modeling also elucidated the structural protection from the stiffened cell cortex, in which 9-15% and 10-19% decrease of maximum stress and strain of the nucleus were obtained. Furthermore, the flat-ended atomic force microscopy probes were used to capture cytoskeleton reorganization after point loading quantitatively, revealing that the larger the applied force and the longer the loading time are, the more pronounced cytoskeleton reorganization is. Also, point loading using a microneedle combined with real-time confocal microscopy uncovered the fast dynamics of actin cytoskeleton reorganization for actin-stained live cells after point loading (<10 s). These results furthered the understandings in the transmission of localized mechanical forces into an adherent cell. |
| DOI | 10.1016/j.bpj.2019.09.012 |
| Indexed By | SCI ; EI |
| Language | 英语 |
| WOS ID | WOS:000490144100004 |
| WOS Keyword | ATOMIC-FORCE MICROSCOPY ; INDUCED CYTOSKELETAL REORGANIZATION ; CELL STIFFNESS ; CYTOCHALASIN-D ; ENDOTHELIAL-CELLS ; PLASMA-MEMBRANE ; CANCER-CELLS ; STRESS ; DECONDENSATION ; ORGANIZATION |
| WOS Research Area | Biophysics |
| WOS Subject | Biophysics |
| Funding Project | National Key Research and Development Program of China[2016YFA0501601] ; National Natural Science Foundation of China[31627804] ; National Natural Science Foundation of China[91642203] ; National Natural Science Foundation of China[31661143044] ; National Natural Science Foundation of China[31570942] ; Frontier Science Key Project of Chinese Science Academy[QYZDJ-SSW-JSC018] ; Strategic Priority Research Program of Chinese Academy of Sciences[XDB22040101] |
| Funding Organization | National Key Research and Development Program of China ; National Natural Science Foundation of China ; Frontier Science Key Project of Chinese Science Academy ; Strategic Priority Research Program of Chinese Academy of Sciences |
| Classification | 一类/力学重要期刊 |
| Ranking | 1 |
| Contributor | Lu, Shouqin ; Long, Mian |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/80670 |
| Collection | 微重力重点实验室 |
| Affiliation | 1.Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Key Lab Micrograv,Natl Micrograv Lab,Beijing Key, Beijing, Peoples R China; 2.Chinese Acad Sci, CAS Ctr Excellence Complex Syst Mech, Inst Mech, Beijing, Peoples R China; 3.Univ Chinese Acad Sci, Sch Engn Sci, Beijing, Peoples R China; 4.Guizhou Med Univ, Key Lab Biol & Med Engn, Immune Cells & Antibody Engn Res Ctr Guizhou Prov, Guiyang, Guizhou, Peoples R China |
| Recommended Citation GB/T 7714 | Hu JR,Chen SB,Hu WH,et al. Mechanical Point Loading Induces Cortex Stiffening and Actin Reorganization[J]. BIOPHYSICAL JOURNAL,2019,117,8,:1405-1418.Rp_Au:Lu, Shouqin, Long, Mian |
| APA | 胡锦荣,陈深宝,胡文慧,吕守芹,&龙勉.(2019).Mechanical Point Loading Induces Cortex Stiffening and Actin Reorganization.BIOPHYSICAL JOURNAL,117(8),1405-1418. |
| MLA | 胡锦荣,et al."Mechanical Point Loading Induces Cortex Stiffening and Actin Reorganization".BIOPHYSICAL JOURNAL 117.8(2019):1405-1418. |
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| Jp2019397.pdf（4950KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
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