| Auto Micro Atomization Delivery of Human Epidermal Organoids Improves Therapeutic Effects for Skin Wound Healing | |
Chang MY1,2; Liu J2; Guo BL1; Fang X(方新)3 ; Wang Y1; Wang SY1,4; Liu XF5; Reid LM6,7; Wang YF1,2
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| Corresponding Author | Wang, Yunfang([email protected]) |
| Source Publication | FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
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| 2020-02-21 | |
| Volume | 8Pages:16 |
| ISSN | 2296-4185 |
| Abstract | Severe skin wounds are often associated with large areas of damaged tissue, resulting in substantial loss of fluids containing electrolytes and proteins. The net result is a vulnerability clinically to skin infections. Therapies aiming to close these large openings are effective in reducing the complications of severe skin wounds. Recently, cell transplantation therapy showed the potential for rapid re-epithelialization of severe skin wounds. Here, we show the improved effects of cell transplantation therapy using a robust protocol of efficient expansion and delivery of epidermal cells for treatment of severe skin wounds. Human skin tissues were used to generate human epidermal organoids maintained under newly established culture conditions. The human epidermal organoids showed an improved capacity of passaging for at least 10 rounds, enabling organoids to expand to cell numbers required for clinical applications. A newly designed auto micro-atomization device (AMAD) was developed for delivery of human epidermal organoids onto the sites of severe skin wounds enhancing uniform and concentrated delivery of organoids, facilitating their engraftment and differentiation for skin reconstitution. With the optimal design and using pneumatic AMAD, both survival and functions of organoids were effectively protected during the spraying process. Cells in the sprayed human epidermal organoids participated in the regeneration of the epidermis at wound sites in a mouse model and accelerated wound healing significantly. The novel AMAD and out new protocol with enhanced effects with respect to both organoid expansion and efficient transplantation will be used for clincal treatments of complex, uneven, or large-area severe skin wounds. |
| Keyword | cell delivery auto micro atomization device (AMAD) cell therapy wound healing organoids |
| DOI | 10.3389/fbioe.2020.00110 |
| Indexed By | SCI ; EI |
| Language | 英语 |
| WOS ID | WOS:000523489100001 |
| WOS Keyword | CELL DELIVERY ; BURNS ; KERATINOCYTES ; EPITHELIALIZATION ; FIBROBLASTS ; MULTICENTER ; EPITHELIUM |
| WOS Research Area | Biotechnology & Applied Microbiology ; Science & Technology - Other Topics |
| WOS Subject | Biotechnology & Applied Microbiology ; Multidisciplinary Sciences |
| Funding Project | National Natural Science Foundations of China[81730052] ; Interdisciplinary Cooperation Project of Beijing Nova Program[Z1811100006218127] ; National Major Scientific and Technological Special Project for Significant New Drugs Development[2018ZX09711003-001-002] ; National Key Research and Development Program of China[2016YFC1101305] |
| Funding Organization | National Natural Science Foundations of China ; Interdisciplinary Cooperation Project of Beijing Nova Program ; National Major Scientific and Technological Special Project for Significant New Drugs Development ; National Key Research and Development Program of China |
| Classification | 一类 |
| Ranking | 4 |
| Contributor | Wang, Yunfang |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/81820 |
| Collection | 非线性力学国家重点实验室 |
| Affiliation | 1.Inst Hlth Serv & Transfus Med, Stem Cell & Tissue Engn Lab, Beijing, Peoples R China; 2.Tsinghua Univ, Beijing Tsinghua Changgung Hosp, Translat Res Ctr, Beijing, Peoples R China; 3.Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech, Beijing, Peoples R China; 4.Chinese Peoples Liberat Army Gen Hosp, Inst TB Res, Army TB Prevent & Control Key Lab, Med Ctr 8, Beijing, Peoples R China; 5.Chinese Peoples Liberat Army PLA, Dept Obstet & Gynecol, Air Force Med Ctr, Beijing, Peoples R China; 6.Univ N Carolina, Dept Cell Biol & Physiol, Sch Med, Chapel Hill, NC 27515 USA; 7.Univ N Carolina, Program Mol Biol & Biotechnol, Sch Med, Chapel Hill, NC 27515 USA |
| Recommended Citation GB/T 7714 | Chang MY,Liu J,Guo BL,et al. Auto Micro Atomization Delivery of Human Epidermal Organoids Improves Therapeutic Effects for Skin Wound Healing[J]. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,2020,8:16.Rp_Au:Wang, Yunfang |
| APA | Chang MY.,Liu J.,Guo BL.,方新.,Wang Y.,...&Wang YF.(2020).Auto Micro Atomization Delivery of Human Epidermal Organoids Improves Therapeutic Effects for Skin Wound Healing.FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,8,16. |
| MLA | Chang MY,et al."Auto Micro Atomization Delivery of Human Epidermal Organoids Improves Therapeutic Effects for Skin Wound Healing".FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY 8(2020):16. |
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| Jp2020117.pdf（2360KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
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