Mechanomics analysis of hESCs under combined mechanical shear, stretch, and compression

doi:10.1007/s10237-020-01378-5

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	Mechanomics analysis of hESCs under combined mechanical shear, stretch, and compression
	Zhang F(张帆)1,2,3 ; Wang JW(王家文)1,2,3; Lv DY(吕东媛)1,2,3 ; Zheng L(郑璐)1,2,3; Shang GB(上官冰)1,2; Gao YX(高宇欣)1,2 ; Wu Y(武亿)1,2,3; Long M(龙勉)1,2,3
通讯作者	Long, Mian([email protected])
发表期刊	BIOMECHANICS AND MODELING IN MECHANOBIOLOGY
	2020-08-18
页码	18
ISSN	1617-7959
摘要	Human embryonic stem cells (hESCs) can differentiate to three germ layers within biochemical and biomechanical niches. The complicated mechanical environments in vivo could have diverse effects on the fate decision and biological functions of hESCs. To globally screen mechanosensitive molecules, three typical types of mechanical stimuli,i.e.,tensile stretch, shear flow, and mechanical compression, were applied in respective parameter sets of loading pattern, amplitude, frequency, and/or duration, and then, iTRAQ proteomics test was used for identifying and quantifying differentially expressed proteins in hESCs. Bioinformatics analysis identified 37, 41, and 23 proteins under stretch pattern, frequency, and duration, 13, 18, and 41 proteins under shear pattern, amplitude, and duration, and 4, 0, and 183 proteins under compression amplitude, frequency, and duration, respectively, where distinct parameters yielded the differentially weighted preferences under each stimulus. Ten mechanosensitive proteins were commonly shared between two of three mechanical stimuli, together with numerous proteins identified under single stimulus. More importantly, functional GSEA and WGCNA analyses elaborated the variations of the screened proteins with loading parameters. Common functions in protein synthesis and modification were identified among three stimuli, and specific functions were observed in skin development under stretch alone. In conclusion, mechanomics analysis is indispensable to map actual mechanosensitive proteins under physiologically mimicking mechanical environment, and sheds light on understanding the core hub proteins in mechanobiology.
关键词	Mechanomics hESCs iTRAQ GSEA WGCNA
DOI	10.1007/s10237-020-01378-5
收录类别	SCI ; EI
语种	英语
WOS记录号	WOS:000560629600001
关键词[WOS]	EMBRYONIC STEM-CELLS ; NUCLEAR-LOCALIZATION ; ARTICULAR-CARTILAGE ; STRESS ; DIFFERENTIATION ; EXPRESSION ; EXPANSION ; FORCES ; MORPHOGENESIS ; PROLIFERATION
WOS研究方向	Biophysics ; Engineering
WOS类目	Biophysics ; Engineering, Biomedical
资助项目	National Natural Science Foundation of China[31661143044] ; National Natural Science Foundation of China[31627804] ; National Natural Science Foundation of China[31870931] ; National Natural Science Foundation of China[31470907] ; Frontier Science Key Project of Chinese Science Academy grant[QYZDJ-SSW-JSC018]
项目资助者	National Natural Science Foundation of China ; Frontier Science Key Project of Chinese Science Academy grant
论文分区	二类
力学所作者排名	1
RpAuthor	Long, Mian
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/84972
专题	微重力重点实验室
作者单位	1.Chinese Acad Sci, Ctr Biomech & Bioengn, Key Lab Micrograv, Natl Micrograv Lab, Beijing 100190, Peoples R China; 2.Chinese Acad Sci, Beijing Key Lab Engn Construct & Mechanobiol, Inst Mech, Beijing 100190, Peoples R China; 3.Univ Chinese Acad Sci, Sch Engn Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhang F,Wang JW,Lv DY,et al. Mechanomics analysis of hESCs under combined mechanical shear, stretch, and compression[J]. BIOMECHANICS AND MODELING IN MECHANOBIOLOGY,2020:18.Rp_Au:Long, Mian
APA	张帆.,王家文.,吕东媛.,郑璐.,上官冰.,...&龙勉.(2020).Mechanomics analysis of hESCs under combined mechanical shear, stretch, and compression.BIOMECHANICS AND MODELING IN MECHANOBIOLOGY,18.
MLA	张帆,et al."Mechanomics analysis of hESCs under combined mechanical shear, stretch, and compression".BIOMECHANICS AND MODELING IN MECHANOBIOLOGY (2020):18.

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