Fluid flow induced calcium response in bone cell network

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	Fluid flow induced calcium response in bone cell network
	Huo B(霍波); Lu XL ; Guo XE
	2009
摘要	In vitro bone cell networks with controlled pattern and intercellular gap junctions were successfully established by using microcontact printing and self assembled monolayers technologies. The intracellular calcium responses of bone cell networks exposed to a steady fluid flow and the underlying signaling pathways were further investigated. The cell network samples were separated into eight groups for treatment with specific pharmacological agents that inhibit pathways significant in bone cell calcium signaling. The calcium transients of the network were recorded and quantitatively evaluated with a set of network parameters. The results showed that gap junction blocker, extracellular ATP pathway inhibitor, and thapsigargin (depleting intracellular calcium stores) significantly reduced the occurrence of multiple calcium peaks, which were visually obvious in the untreated group. The number of responsive peaks also decreased slightly yet significantly when either the COX-2/PGE2 or the NOS/nitric oxide pathway was disrupted. In the absence of calcium in the culture medium, the intracellular calcium concentration decreased slowly with fluid flow without any calcium transients observed. These findings have identified important factors in the flow mediated calcium signaling of bone cells within an in vitro network. © 2010 by World Scientific Publishing Co. Pte. Ltd. All rights reserved.
专著/编著/文集名	Tributes to Yuan-Cheng Fung on his 90th Birthday: Biomechanics: From Molecules to Man
页码	127-142
出版者	World Scientific Publishing Co.
语种	英语
ISBN号	9789814289955
文献类型	图书章节
条目标识符	http://dspace.imech.ac.cn/handle/311007/85082
专题	微重力重点实验室
作者单位	1.Bone Bioengineering Laboratory, Department of Bioengineering, Columbia University, New York, NY 10027, United States 2.Institute of Mechanics, Chinese Academy of Sciences, Beijing, 100080, China
推荐引用方式 GB/T 7714	Huo B,Lu XL,Guo XE. Fluid flow induced calcium response in bone cell network[Ch]. Tributes to Yuan-Cheng Fung on his 90th Birthday: Biomechanics: From Molecules to Man,World Scientific Publishing Co.,2009.

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