Regulation of hepatic differentiation of human embryonic stem cells by calcium silicate extracts for liver injury repairing

doi:10.1016/j.apmt.2020.100730

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	Regulation of hepatic differentiation of human embryonic stem cells by calcium silicate extracts for liver injury repairing
	Zheng L; Lv DY(吕东媛); Zhang F; Xing M; Wang XY; Jia XH; Shu XY; Li PW(李培文); Lv SQ(吕守芹); Chang J; Long M(龙勉)
发表期刊	Applied Materials Today
	2020-09-01
卷号	20 页码:100730
ISSN	2352-9407
摘要	Directing embryonic stem cell (ESC)-derived hepatocytes is critical in understanding hepatic differentiation and applying cell-based treatment to severe liver diseases. While growth factor-based strategies are widely used, using chemical cues could present an alternative to optimize the strategies for stem cell differentiation. Here, for the first time, an inorganic calcium silicate (CS, CaSiO3)-based approach, together with a modified four-stage differentiating protocol, was proposed to quantify the effects of CS extracts on inducing hepatic differentiation of human ESCs (H9 cells). The roles of CS-activated H9 cells in liver injury repair were tested by cell tracking and immunohistochemical staining. Results indicated that high concentrations of CS extracts initially enhance definitive endodermal (DE) lineage, followed by gradual DE differentiation at low CS concentrations. The order of CS addition is also crucial, since the presence at stemness stage and the absence at DE stage could optimize hepatic differentiation capacity of H9 cells, resulting in optimized cells that differentiate into functional hepatocyte-like cells. The addition of CS extracts at precursor hepatocyte stage enhances their maturity, which favors the turnover of liver injury in CCl4-treated mice. These results provide an insight into applying bioactive inorganic biomaterials to foster hepatic differentiation of human ESCs for cell therapy.
关键词	Human embryonic stem cells Hepatic differentiation Hepatocyte-like cells Calcium silicate extracts Liver injury repair
DOI	10.1016/j.apmt.2020.100730
收录类别	SCI ; EI
语种	英语
WOS记录号	WOS:000598353800004
论文分区	一类
力学所作者排名	1
RpAuthor	Jiang Chang, Mian Long
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://dspace.imech.ac.cn/handle/311007/85682
专题	微重力重点实验室
推荐引用方式 GB/T 7714	Zheng L,Lv DY,Zhang F,et al. Regulation of hepatic differentiation of human embryonic stem cells by calcium silicate extracts for liver injury repairing[J]. Applied Materials Today,2020,20:100730.Rp_Au:Jiang Chang, Mian Long
APA	Zheng L.,吕东媛.,Zhang F.,Xing M.,Wang XY.,...&龙勉.(2020).Regulation of hepatic differentiation of human embryonic stem cells by calcium silicate extracts for liver injury repairing.Applied Materials Today,20,100730.
MLA	Zheng L,et al."Regulation of hepatic differentiation of human embryonic stem cells by calcium silicate extracts for liver injury repairing".Applied Materials Today 20(2020):100730.

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