| Global mapping of live cell mechanical features usingPeakForce QNM AFM | |
Hu JR(胡锦荣) ; Chen SB(陈深宝); Huang DD(黄丹丹); Zhang Y(章燕); Lv SQ(吕守芹); Long M(龙勉)
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| Source Publication | Biophysics Reports
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| 2020-01-22 | |
| Volume | 6Issue:1Pages:9-18 |
| Abstract | Atomic force microscopy (AFM) has been widely used to acquire surface topography upon different scanning modes and to quantify mechanical properties of a cell using single-point ramp force mode. However, these traditional measurements need massive force curves originating from multiple points of a cell to exclude the potential errors resulted from limited and factitious selections of testing points, making the measurements time-consuming and highly localized. PeakForce Quantitative NanoMechanics (PF QNM) is a high-speed (faster by 3–4 order of magnitude) and global surface mechanical property mapping method with high spatial resolution, overcoming the drawbacks of traditional ramp mode especially used for a live cell with high heterogeneity. In this protocol, we elaborated how to run PF QNM measurements for live cells and relevant modification may be needed when extending this method to other cell-like soft materials. |
| DOI | 10.1007/s41048-019-00103-9 |
| Indexed By | 其他 |
| Language | 英语 |
| Classification | 其他 |
| Ranking | 1 |
| Contributor | Shouqin Lü, Mian Long |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/85684 |
| Collection | 微重力重点实验室 |
| Recommended Citation GB/T 7714 | Hu JR,Chen SB,Huang DD,et al. Global mapping of live cell mechanical features usingPeakForce QNM AFM[J]. Biophysics Reports,2020,6,1,:9-18.Rp_Au:Shouqin Lü, Mian Long |
| APA | 胡锦荣,陈深宝,黄丹丹,章燕,吕守芹,&龙勉.(2020).Global mapping of live cell mechanical features usingPeakForce QNM AFM.Biophysics Reports,6(1),9-18. |
| MLA | 胡锦荣,et al."Global mapping of live cell mechanical features usingPeakForce QNM AFM".Biophysics Reports 6.1(2020):9-18. |
| Files in This Item: | Download All | |||||
| File Name/Size | DocType | Version | Access | License | ||
| Jp2020554.pdf（2838KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
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