| Hepatic differentiation of human embryonic stem cells by coupling substrate stiffness and microtopography | |
Luo CH(罗春花)1,2; Lv DY(吕东媛)1,2,3 ; Zheng L(郑鲁)1,2,3; Zhang F(张帆)1,2,3; Zhang X(张潇)1,2,3; Lv SQ(吕守芹)1,2,3; Zhang C(张晨)1,2; Jia Xiaohua4; Shu XY(舒芯钰)1,2,3; Li PW(李培文)1,2; Li Z(李展)1,2; Long M(龙勉)1,2,3
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| Corresponding Author | Long, Mian([email protected]) |
| Source Publication | BIOMATERIALS SCIENCE
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| 2021-04-20 | |
| Pages | 15 |
| ISSN | 2047-4830 |
| Abstract | Mechanical or physical cues are associated with the growth and differentiation of embryonic stem cells (ESCs). While the substrate stiffness or topography independently affects the differentiation of ESCs, their cooperative regulation on lineage-specific differentiation remains largely unknown. Here, four topographical configurations on stiff or soft polyacrylamide hydrogel were combined to direct hepatic differentiation of human H1 cells via a four-stage protocol, and the coupled impacts of stiffness and topography were quantified at distinct stages. Data indicated that the substrate stiffness is dominant in stemness maintenance on stiff gel and hepatic differentiation on soft gel while substrate topography assists the differentiation of hepatocyte-like cells in positive correlation with the circularity of H1 clones initially formed on the substrate. The differentiated cells exhibited liver-specific functions such as maintaining the capacities of CYP450 metabolism, glycogen synthesis, ICG engulfment, and repairing liver injury in CCl4-treated mice. These results implied that the coupling of substrate stiffness and topography, combined with the biochemical signals, is favorable to improve the efficiency and functionality of hepatic differentiation of human ESCs. |
| DOI | 10.1039/d1bm00174d |
| Indexed By | SCI ; EI |
| Language | 英语 |
| WOS ID | WOS:000641302300001 |
| WOS Research Area | Materials Science |
| WOS Subject | Materials Science, Biomaterials |
| Funding Project | National Natural Science Foundation of China[91642203] ; National Natural Science Foundation of China[31627804] ; National Natural Science Foundation of China[31870931] ; National Natural Science Foundation of China[31661143044] ; National Natural Science Foundation of China[31470907] ; National Natural Science Foundation of China[81703012] ; Chinese Science Academy[QYZDJ-SSW-JSC018] ; Chinese Science Academy[GJJSTU20190005] ; Chinese Science Academy[XDB22040101] |
| Funding Organization | National Natural Science Foundation of China ; Chinese Science Academy |
| Classification | 二类/Q1 |
| Ranking | 1 |
| Contributor | Long, Mian |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/86480 |
| Collection | 微重力重点实验室 |
| Affiliation | 1.Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Key Lab Micrograv,Natl Micrograv Lab, Beijing 100190, Peoples R China; 2.Chinese Acad Sci, Inst Mech, Beijing Key Lab Engn Construct & Mechanobiol, Beijing 100190, Peoples R China; 3.Univ Chinese Acad Sci, Sch Engn Sci, Beijing 100049, Peoples R China; 4.Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, Beijing 100190, Peoples R China |
| Recommended Citation GB/T 7714 | Luo CH,Lv DY,Zheng L,et al. Hepatic differentiation of human embryonic stem cells by coupling substrate stiffness and microtopography[J]. BIOMATERIALS SCIENCE,2021:15.Rp_Au:Long, Mian |
| APA | 罗春花.,吕东媛.,郑鲁.,张帆.,张潇.,...&龙勉.(2021).Hepatic differentiation of human embryonic stem cells by coupling substrate stiffness and microtopography.BIOMATERIALS SCIENCE,15. |
| MLA | 罗春花,et al."Hepatic differentiation of human embryonic stem cells by coupling substrate stiffness and microtopography".BIOMATERIALS SCIENCE (2021):15. |
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| Jp2021F166.pdf（7505KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
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