The fatigue tests of Chinese RPV steel (A508-3) were carried out under the simulated primary coolant environment of AP1000 which is a kind of 3rd generation nuclear power plant. The effects of temperature, strain rate and dissolved oxygen (DO) on fatigue life were analyzed. During the fatigue test, the material exhibited a cyclic hardening followed by cyclic softening behavior, with no obvious cyclic stability region. In logarithmic coordinates, the S-N curve showed a bilinear relationship. As the strain amplitude increased from 0.2 to 0.6%, the fatigue life approximately decreased from 10(5) to 3 x 10(2). The environmental correction factor (Fen), which is defined as the ratio of life in air at room temperature to that in the primary coolant environment, decreases as the temperature decreases from 321 to 150 degrees C. The Fen also decreases as the DO decreases from 0.6 to 0.03 ppm while it increases as the strain rate decreases from 1 to 0.0004%/s. Compared with the NUREG6909 model, the presented model is more applicable for fatigue life prediction of Chinese A508-3 steel.