| A gel-like condensation of Cidec generates lipid-permeable plates for lipid droplet fusion | |
Lyu, XC1,2; Wang, J1,2; Wang, JQ1,2 ; Yin, YS3; Zhu, Y4; Li, LL5; Huang, SR6,7,8; Peng S(彭爽)9; Xue, BX4; Liao, RY1,2; Wang, SQ5; Long M(龙勉)9; Wohland, T6,7,8; Chua, BT3; Sun, YJ4; Li, PL10; Chen, XW11,12; Xu, L1,2,13; Chen, FJ3,13; Li, P1,2,13
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| Corresponding Author | Chen, Feng-Jung([email protected]) ; Li, Peng([email protected]) |
| Source Publication | DEVELOPMENTAL CELL
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| 2021-09-27 | |
| Volume | 56Issue:18Pages:2592-+ |
| ISSN | 1534-5807 |
| Abstract | Membrane contact between intracellular organelles is important in mediating organelle communication. However, the assembly of molecular machinery at membrane contact site and its internal organization correlating with its functional activity remain unclear, Here, we demonstrate that a gel-like condensation of Cidec, a crucial protein for obesity development by facilitating lipid droplet (LD) fusion, occurs at the LD-LD contact site (LDCS) through phase separation. The homomeric interaction between the multivalent N terminus of Cidec is sufficient to promote its phase separation both in vivo and in vitro. Interestingly, Cidec condensation at LDCSs generates highly plastic and lipid-permeable fusion plates that are geometrically constrained by donor LDs. In addition, Cidec condensates are distributed unevenly in the fusion plate generating stochastic sub-compartments that may represent unique lipid passageways during LD fusion. We have thus uncovered the organization and functional significance of geometry-constrained Cidec phase separation in mediating LD fusion and lipid homeostasis. |
| DOI | 10.1016/j.devcel.2021.08.015 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000706446300008 |
| WOS Keyword | LIQUID PHASE-SEPARATION ; STRESS GRANULES ; LIVE-CELL ; PROTEIN ; GROWTH ; DNA ; TRANSITION ; MICROSCOPY ; SECRETION ; RESISTANT |
| WOS Research Area | Cell Biology ; Developmental Biology |
| WOS Subject | Cell Biology ; Developmental Biology |
| Funding Project | Tsinghua University ; National Key R&D Program of China[2018YFA050 6901] ; National Key R&D Program of China[2018YFA0800301] ; National Key R&D Program of China[2016YFA0502002] ; National Key R&D Program of China[2019YFA08 01701] ; National Natural Science Foundation of China[91857103] ; National Natural Science Foundation of China[91854104] ; National Natural Science Foundation of China[31690103] ; High Level Medicine Foundation of Shanghai Government ; Shanghai Municipal Science and Technology Major Project[2017SHZDZX01] |
| Funding Organization | Tsinghua University ; National Key R&D Program of China ; National Natural Science Foundation of China ; High Level Medicine Foundation of Shanghai Government ; Shanghai Municipal Science and Technology Major Project |
| Classification | 一类 |
| Ranking | 3+ |
| Contributor | Chen, Feng-Jung ; Li, Peng |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/87624 |
| Collection | 微重力重点实验室 |
| Affiliation | 1.Tsinghua Univ, Beijing Adv Innovat Ctr Struct Biol, Sch Life Sci, State Key Lab Membrane Biol, Beijing 100084, Peoples R China; 2.Tsinghua Univ, Beijing Adv Innovat Ctr Struct Biol, Sch Life Sci, Tsinghua Peking Ctr Life Sci, Beijing 100084, Peoples R China; 3.Fudan Univ, Zhongshan Hosp, Inst Metab & Integrat Biol, Dept Endocrinol & Metab,Shanghai Key Lab Metab Re, Shanghai 200032, Peoples R China; 4.Peking Univ, Biodynam Opt Imaging Ctr BIOPIC, Sch Life Sci, Beijing 100871, Peoples R China; 5.Peking Univ, Coll Life Sci, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100871, Peoples R China; 6.Natl Univ Singapore, Dept Biol Sci, 14 Sci Dr 4, Singapore 117557, Singapore; 7.Natl Univ Singapore, Dept Chem, 14 Sci Dr 4, Singapore 117557, Singapore; 8.Natl Univ Singapore, NUS Ctr Bioimaging Sci, 14 Sci Dr 4, Singapore 117557, Singapore; 9.Chinese Acad Sci, Inst Mech, 15 Beisihuanxi Rd, Beijing 100190, Peoples R China; 10.Tsinghua Univ, Beijing Adv Innovat Ctr Struct Biol, Tsinghua Peking Joint Ctr Life Sci, Sch Life Sci, Beijing 100084, Peoples R China; 11.Peking Univ, Coll Future Technol, Ctr Life Sci, State Key Lab Membrane Biol, Beijing 100101, Peoples R China; 12.Peking Univ, Coll Future Technol, Inst Mol Med, Beijing 100101, Peoples R China; 13.Shanghai Qi Zhi Inst, Shanghai 200030, Peoples R China |
| Recommended Citation GB/T 7714 | Lyu, XC,Wang, J,Wang, JQ,et al. A gel-like condensation of Cidec generates lipid-permeable plates for lipid droplet fusion[J]. DEVELOPMENTAL CELL,2021,56,18,:2592-+.Rp_Au:Chen, Feng-Jung, Li, Peng |
| APA | Lyu, XC.,Wang, J.,Wang, JQ.,Yin, YS.,Zhu, Y.,...&Li, P.(2021).A gel-like condensation of Cidec generates lipid-permeable plates for lipid droplet fusion.DEVELOPMENTAL CELL,56(18),2592-+. |
| MLA | Lyu, XC,et al."A gel-like condensation of Cidec generates lipid-permeable plates for lipid droplet fusion".DEVELOPMENTAL CELL 56.18(2021):2592-+. |
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| Jp2021F535.pdf（4639KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
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