| Tunable and Contamination-Free Injection with Microfluidics by Stepinjection | |
Hu, BY1,2,3; Ye, S1,4; Chen, DW1,3 ; Xie, BL1,2; Hu, R1,3; Qiao, YX1,3; Yu, YH1; Yu, HY1,5; Zheng X(郑旭)6; Lan, Y1; Du, WB1,3,5
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| Corresponding Author | Du, Wenbin([email protected]) |
| Source Publication | ANALYTICAL CHEMISTRY
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| 2021-10-05 | |
| Volume | 93Issue:39Pages:13112-13117 |
| ISSN | 0003-2700 |
| Abstract | Droplet microfluidics with picoinjection provides significant advantages to multistep reactions and screenings. The Tjunction design for picoinjection is convenient in adding picoliter reagents into passing droplets to initiate reactions. However, conventional picoinjectors face difficulties in eliminating cross-contamination between droplets, preventing them from widespread use in sensitive biological and molecular assays. Here, we introduce stepinjection, which uses a T-junction with a stepped channel design to elevate the diffusional buffer zone into the main channel and consequently increases the pressure difference between droplets and the inlet of the injection channel. To demonstrate the stepinjector's ability to perform contamination-sensitive enzymatic assays, we inject casein fluorescein isothiocyanate (FITC-casein) into a mixture of savinase and savinase-free (labeled with a red fluorescent dye) droplets. We observe no cross-contamination using stepinjection but find a severe cross-talk using an optimal picoinjection design. We envision that the simple, tunable, and reliable stepinjector can be easily integrated in various droplet processing devices, and facilitate various biomedical and biochemical applications including multiplex digital PCR, single-cell sequencing, and enzymatic screening. |
| DOI | 10.1021/acs.analchem.1c02721 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000705944200003 |
| WOS Keyword | HIGH-THROUGHPUT ; DROPLET MICROFLUIDICS |
| WOS Research Area | Chemistry |
| WOS Subject | Chemistry, Analytical |
| Funding Project | National Key Research and Development Program of China[2018YFC0310703] ; National Key Research and Development Program of China[2016YFE0205800] ; International Ocean Resource Survey and Exploration[DY135-B-02] ; National Natural Science Foundation of China[21822408] ; National Natural Science Foundation of China[91951103] ; National Natural Science Foundation of China[12072350] ; National Natural Science Foundation of China[11832017] ; National Natural Science Foundation of China[32100073] ; Key Program of Frontier Sciences of the Chinese Academy of Sciences[QYZDB-SSWSMC008] ; Key Program of Frontier Sciences of the Chinese Academy of Sciences[QYZDB-SSW-JSC036] ; CAS Strategic Priority Research Program[XDB22040403] ; Center for Ocean Mega-Science, Chinese Academy of Sciences[KEXUE2019GZ05] |
| Funding Organization | National Key Research and Development Program of China ; International Ocean Resource Survey and Exploration ; National Natural Science Foundation of China ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; CAS Strategic Priority Research Program ; Center for Ocean Mega-Science, Chinese Academy of Sciences |
| Classification | 一类 |
| Ranking | 3+ |
| Contributor | Du, Wenbin |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/87635 |
| Collection | 非线性力学国家重点实验室 |
| Affiliation | 1.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China; 2.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China; 3.Chinese Acad Sci, Inst Microbiol, State Key Lab Transducer Technol, Beijing 100101, Peoples R China; 4.Penn State Univ, Biomed Engn Dept, Coll Engn, University Pk, PA 16802 USA; 5.Univ Chinese Acad Sci, Savaid Med Sch, Beijing 100049, Peoples R China; 6.Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech, Beijing 100190, Peoples R China |
| Recommended Citation GB/T 7714 | Hu, BY,Ye, S,Chen, DW,et al. Tunable and Contamination-Free Injection with Microfluidics by Stepinjection[J]. ANALYTICAL CHEMISTRY,2021,93,39,:13112-13117.Rp_Au:Du, Wenbin |
| APA | Hu, BY.,Ye, S.,Chen, DW.,Xie, BL.,Hu, R.,...&Du, WB.(2021).Tunable and Contamination-Free Injection with Microfluidics by Stepinjection.ANALYTICAL CHEMISTRY,93(39),13112-13117. |
| MLA | Hu, BY,et al."Tunable and Contamination-Free Injection with Microfluidics by Stepinjection".ANALYTICAL CHEMISTRY 93.39(2021):13112-13117. |
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| Jp2021F532.pdf（5316KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
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