| A Novel Human Antibody, HF, against HER2/erb-B2 Obtained by a Computer-Aided Antibody Design Method | |
Qiao, Chunxia1,2; Lv, Ming2,3; Li, Xinying1,2,3; Lang, Xiaoling4; Lv SQ(吕守芹)5 ; Long M(龙勉)5; Li, Yan2,3; Geng, Shusheng4; Lin, Zhou2,3; Shen, Beifen2,3; Feng, Jiannan1,2
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| Corresponding Author | Shen, Beifen([email protected]) ; Feng, Jiannan([email protected]) |
| Source Publication | ENGINEERING
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| 2021-11-01 | |
| Volume | 7Issue:11Pages:1566-1576 |
| ISSN | 2095-8099 |
| Abstract | Fully human antibodies have minimal immunogenicity and safety profiles. At present, most potential antibody drugs in clinical trials are humanized or fully human. Human antibodies are mostly generated using the phage display method (in vitro) or by transgenic mice (in vivo); other methods include B lymphocyte immortalization, human-human hybridoma, and single-cell polymerase chain reaction. Here, we describe a structure-based computer-aided de novo design technology for human antibody generation. Based on the complex structure of human epidermal growth factor receptor 2 (HER2)/Herceptin, we first designed six short peptides targeting the potential epitope of HER2 recognized by Herceptin. Next, these peptides were set as complementarity determining regions in a suitable immunoglobulin frame, giving birth to a novel anti-HER2 antibody named "HF," which possessed higher affinity and more effective anti-tumor activity than Herceptin. Our work offers a useful tool for the quick design and selection of novel human antibodies for basic mechanical research as well as for imaging and clinical applications in immune-related diseases, such as cancer and infectious diseases. (C) 2021 THE AUTHORS. Published by Elsevier LTD on behalf of Chinese Academy of Engineering and Higher Education Press Limited Company. |
| Keyword | HER2/erb-B2 Human antibody Computer-aided design |
| DOI | 10.1016/j.eng.2020.10.024 |
| Indexed By | SCI ; EI |
| Language | 英语 |
| WOS ID | WOS:000738823800011 |
| WOS Keyword | TNF-ALPHA ; COMPUTATIONAL DESIGN ; RECEPTOR ; COMPLEX ; REGION ; HER2 |
| WOS Research Area | Engineering |
| WOS Subject | Engineering, Multidisciplinary |
| Funding Project | National Sciences Fund[31370938] ; National Sciences Fund[81272528] |
| Funding Organization | National Sciences Fund |
| Classification | 一类 |
| Ranking | 3+ |
| Contributor | Shen, Beifen ; Feng, Jiannan |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/88294 |
| Collection | 微重力重点实验室 |
| Affiliation | 1.Inst Pharmacol & Toxicol, State Key Lab Toxicol & Med Countermeasures, Beijing 100850, Peoples R China; 2.Beijing Key Lab Therapeut Gene Engn Antibody, Beijing 100850, Peoples R China; 3.Inst Mil Cognit & Brain Sci, Beijing 100850, Peoples R China; 4.Beijing Mabworks Biotech Co Ltd, Beijing 100176, Peoples R China; 5.Chinese Acad Sci, Inst Mech, Beijing 100190, Peoples R China |
| Recommended Citation GB/T 7714 | Qiao, Chunxia,Lv, Ming,Li, Xinying,et al. A Novel Human Antibody, HF, against HER2/erb-B2 Obtained by a Computer-Aided Antibody Design Method[J]. ENGINEERING,2021,7,11,:1566-1576.Rp_Au:Shen, Beifen, Feng, Jiannan |
| APA | Qiao, Chunxia.,Lv, Ming.,Li, Xinying.,Lang, Xiaoling.,吕守芹.,...&Feng, Jiannan.(2021).A Novel Human Antibody, HF, against HER2/erb-B2 Obtained by a Computer-Aided Antibody Design Method.ENGINEERING,7(11),1566-1576. |
| MLA | Qiao, Chunxia,et al."A Novel Human Antibody, HF, against HER2/erb-B2 Obtained by a Computer-Aided Antibody Design Method".ENGINEERING 7.11(2021):1566-1576. |
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| A Novel Human Antibo（4174KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
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