| Stiff Extracellular Matrix Promotes Invasive Behaviors of Trophoblast Cells | |
| Cao, Jialing1; Li HY(李航宇); Tang, Hongyan; Gu, Xuenan; Wang, Yan4; Guan DS(关东石); Du, Jing; Fan, Yubo | |
| Source Publication | BIOENGINEERING BASEL
 |
| 2023-03 | |
| Volume | 10Issue:3Pages:384 |
| Abstract | The effect of extracellular matrix (ECM) stiffness on embryonic trophoblast cells invasion during mammalian embryo implantation remains largely unknown. In this study, we investigated the effects of ECM stiffness on various aspects of human trophoblast cell behaviors during cell ECM interactions. The mechanical microenvironment of the uterus was simulated by fabricating polyacrylamide (PA) hydrogels with different levels of stiffness. The human choriocarcinoma (JAR) cell lineage was used as the trophoblast model. We found that the spreading area of JAR cells, the formation of focal adhesions, and the polymerization of the F actin cytoskeleton were all facilitated with increased ECM stiffness. Significantly, JAR cells also exhibited durotactic behavior on ECM with a gradient stiffness. Meanwhile, stiffness of the ECM affects the invasion of multicellular JAR spheroids. These results demonstrated that human trophoblast cells are mechanically sensitive, while the mechanical properties of the uterine microenvironment could play an important role in the implantation process. |
| Keyword | embryo implantation human choriocarcinoma cell extracellular matrix stiffness durotaxis |
| DOI | 10.3390/bioengineering10030384 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000957330500001 |
| WOS Research Area | Biotechnology & Applied Microbiology ; Engineering, Biomedical |
| WOS Subject | Biotechnology & Applied Microbiology ; Engineering |
| Funding Organization | National Natural Science Foundation of China [12222201, 82273500, U20A20390, 11827803, 11972351] ; Fundamental Research Funds for the Central Universities [ZG140S1971] ; National Key R&D Program of China [2021YFA0719302] ; Peking University Health Science Center X Cross Seed Fund [BMU2020MX014] ; Incubation fund for reserve candidates of Peking University Third Hospital [BYSYFY2021007] |
| Classification | 二类 |
| Ranking | 1 |
| Contributor | Du, J ; Fan, YB (corresponding author), Beihang Univ, Inst Nanotechnol Single Cell Anal, Beijing Adv Innovat Ctr Biomed Engn, Sch Biol Sci & Med Engn,Minist Educ,Key Lab Biomec, Beijing 100083, Peoples R China. ; Guan, DS (corresponding author), Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech, Beijing 100190, Peoples R China. ; Guan, DS (corresponding author), Univ Chinese Acad Sci, Sch Engn Sci, Beijing 100049, Peoples R China. ; Wang, Y (corresponding author), Peking Univ Third Hosp, Dept Obstet & Gynecol, Beijing 100191, Peoples R China. |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/91791 |
| Collection | 非线性力学国家重点实验室 |
| Affiliation | 1.Beihang Univ, Inst Nanotechnol Single Cell Anal, Beijing Adv Innovat Ctr Biomed Engn, Sch Biol Sci & Med Engn,Minist Educ,Key Lab Biomec, Beijing 100083, Peoples R China 2.Beihang Univ, Sino French Engineer Sch, Beijing 100083, Peoples R China 3.Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech, Beijing 100190, Peoples R China 4.Univ Chinese Acad Sci, Sch Engn Sci, Beijing 100049, Peoples R China 5.Peking Univ Third Hosp, Dept Obstet & Gynecol, Beijing 100191, Peoples R China |
| Recommended Citation GB/T 7714 | Cao, Jialing,Li HY,Tang, Hongyan,et al. Stiff Extracellular Matrix Promotes Invasive Behaviors of Trophoblast Cells[J]. BIOENGINEERING BASEL,2023,10,3,:384.Rp_Au:Du, J, Fan, YB (corresponding author), Beihang Univ, Inst Nanotechnol Single Cell Anal, Beijing Adv Innovat Ctr Biomed Engn, Sch Biol Sci & Med Engn,Minist Educ,Key Lab Biomec, Beijing 100083, Peoples R China., Guan, DS (corresponding author), Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech, Beijing 100190, Peoples R China., Guan, DS (corresponding author), Univ Chinese Acad Sci, Sch Engn Sci, Beijing 100049, Peoples R China., Wang, Y (corresponding author), Peking Univ Third Hosp, Dept Obstet & Gynecol, Beijing 100191, Peoples R China. |
| APA | Cao, Jialing.,李航宇.,Tang, Hongyan.,Gu, Xuenan.,Wang, Yan.,...&Fan, Yubo.(2023).Stiff Extracellular Matrix Promotes Invasive Behaviors of Trophoblast Cells.BIOENGINEERING BASEL,10(3),384. |
| MLA | Cao, Jialing,et al."Stiff Extracellular Matrix Promotes Invasive Behaviors of Trophoblast Cells".BIOENGINEERING BASEL 10.3(2023):384. |
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| Jp2023A007.pdf（26194KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
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