| Comparison of modified molecules 3 aminopropyltriethoxysilane and 11 aminoundecyltriethoxysilane in orientation angle and interaction with protein by sum frequency vibration spectrum and imaging ellipsometry biosensor | |
Liu HY(刘浩雨); Jin G(靳刚)
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| Source Publication | THIN SOLID FILMS
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| 2023-03-31 | |
| Volume | 769Pages:139738 |
| ISSN | 0040-6090 |
| Abstract | 3 Aminopropyltriethoxysilane (APTES) has been widely used in biosensing as a surface modifying molecule that acts as a bridge. The orientation and structure of APTES at the interface are major factors that affect biosensing efficiency. IgG (immunoglobulin G) is the most used and one of the most abundant immune proteins in the serum. We not only used broadband sum frequency generation vibrational spectroscopy to compare the spectrum of APTES and 11 aminoundecyltriethoxysilane (AUTES) molecules modified on a Si (111) surface but also compared the number and sensitivity of IgG adhered on APTES and AUTES surfaces by the imaging ellipsometry biosensor. We first found that the terminal amino groups of the APTES molecule were disorderly arranged by Sum frequency vibration spectrum (SFVS). Then we compared the performance of IgG immobilization in APTES or AUTES surfaces by imaging ellipsometry biosensor (IEB). In IEB, the anti IgG detection LOD (limit of detec tion) on the APTES modified surface(51 ng/mL) was higher than that on the AUTES modified surface(380 ng/ mL). We explored the relationship between the stability of aminosilane and the stability of biomolecules, and provided a reference for the selection of surface modification methods using aminosilane as a self assembled membrane. |
| Keyword | Aminosilane Surface modification Molecular orientation angle Specificity Immune globulin Imaging ellipsometry biosensor Sum frequency vibration spectrum |
| DOI | 10.1016/j.tsf.2023.139738 |
| Indexed By | SCI ; EI |
| Language | 英语 |
| WOS ID | WOS:000948432900001 |
| WOS Research Area | Materials Science, Multidisciplinary ; Materials Science, Coatings & Films ; Physics, Applied ; Physics, Condensed Matter |
| WOS Subject | Materials Science ; Physics |
| Classification | Q3 |
| Ranking | 1 |
| Contributor | Jin, G (corresponding author), Chinese Acad Sci, Inst Mech, NML, 15 Bei Si Huan West Rd, Beijing 100190, Peoples R China. |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://dspace.imech.ac.cn/handle/311007/91816 |
| Collection | 微重力重点实验室 |
| Affiliation | 1.Chinese Acad Sci, Inst Mech, NML, 15 Bei Si Huan West Rd, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Sch Engn Sci, 19 Yu Quan Rd, Beijing 100049, Peoples R China 3.Univ Chinese Acad Sci, Hangzhou Inst Adv Study, Sch Fundamental Phys & Math Sci, 1 Sub Lane Xiangshan, Hangzhou 310024, Peoples R China |
| Recommended Citation GB/T 7714 | Liu HY,Jin G. Comparison of modified molecules 3 aminopropyltriethoxysilane and 11 aminoundecyltriethoxysilane in orientation angle and interaction with protein by sum frequency vibration spectrum and imaging ellipsometry biosensor[J]. THIN SOLID FILMS,2023,769:139738.Rp_Au:Jin, G (corresponding author), Chinese Acad Sci, Inst Mech, NML, 15 Bei Si Huan West Rd, Beijing 100190, Peoples R China. |
| APA | 刘浩雨,&靳刚.(2023).Comparison of modified molecules 3 aminopropyltriethoxysilane and 11 aminoundecyltriethoxysilane in orientation angle and interaction with protein by sum frequency vibration spectrum and imaging ellipsometry biosensor.THIN SOLID FILMS,769,139738. |
| MLA | 刘浩雨,et al."Comparison of modified molecules 3 aminopropyltriethoxysilane and 11 aminoundecyltriethoxysilane in orientation angle and interaction with protein by sum frequency vibration spectrum and imaging ellipsometry biosensor".THIN SOLID FILMS 769(2023):139738. |
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| Jp2023Fa269.pdf（2023KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
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